What is the malignancy risk in patients with rheumatologic disease being treated with either a TNF inhibitor (such as adalimumab), JAK inhibitor (such as tofacitinib), or IL-6 inhibitor (such as tocilizumab)?

Comment by InpharmD Researcher

Current evidence indicates that the malignancy risk in patients with rheumatologic diseases treated with tumor necrosis factor (TNF) inhibitors, Janus kinase (JAK) inhibitors, or interleukin-6 (IL-6) inhibitors is generally low, although specific rates across studies vary depending on the agent and comparator. Large meta-analyses show that TNF inhibitors do not significantly increase cancer risk, with incidence rates around 1.0 to 3.2 per 100 patient-years. Pooled findings also suggest that JAK inhibitors, including tofacitinib, do not significantly alter overall cancer risk, although some analyses indicate a modestly higher risk compared with TNF inhibitors, with lymphoma incidence slightly elevated in long-term trials. IL-6 inhibition with tocilizumab does not appear to increase overall cancer risk, with pooled relative risk estimates near 1.0. Notably, a recent analysis found no increased risk of overall cancer or non-melanoma skin cancer for IL-6 inhibitors or JAK inhibitors compared with TNF inhibitors, supporting a low absolute risk across these therapies (see Table 1).

Background

A 2025 systematic review and meta-analysis examined the comparative safety profiles of Janus kinase (JAK) inhibitors versus tumor necrosis factor (TNF) antagonists in patients with immune-mediated inflammatory diseases (IMIDs). The investigation encompassed 42 head-to-head trials (N= 813,881), involving participants with different types of IMIDs, including rheumatoid arthritis, inflammatory bowel disease, psoriasis or psoriatic arthritis, and spondyloarthropathy. Hazard ratios and incidence rates were calculated for safety outcomes which included serious infections, malignant neoplasms, major cardiovascular events (MACEs), and venous thromboembolism (VTE) . The results revealed no significant differences in the risk of serious infections (incidence rate of 3.79 vs 3.03 per 100 person-years), malignant neoplasms (1.00 vs 0.94 per 100 person-years), or MACEs (0.72 vs 0.66 per 100 person-years) between users of JAK inhibitors and TNF antagonists. However, the study observed a slightly heightened risk of VTE associated with JAK inhibitors, reflected by a pooled hazard ratio of 1.26 compared to TNF antagonists. These findings, consistent across subgroups, highlight a low overall incidence of serious adverse events in this therapeutic area; however, they suggest a call for regulatory bodies to reconsider existing restrictions on JAK inhibitors. [1]

A 2023 meta-analysis evaluating cancer risk in patients treated with the JAK inhibitor tofacitinib included 22 articles describing 26 controlled studies, of which 22 were randomized controlled trials. When comparing tofacitinib with any control treatment, the relative risk (RR) for any cancer was 1.06 (95% confidence interval [CI] 0.86 to 1.31; p = 0.95), indicating no significant difference. In separate analyses comparing tofacitinib to either placebo or biological therapy, there was also no significant difference in overall cancer risk, with an RR of 1.04 (95% CI 0.44 to 2.48; p= 0.95) versus placebo and an RR of 1.06 (95% CI 0.86 to 1.31; p= 0.58) versus biological drugs. However, when tofacitinib was compared specifically with tumor necrosis factor (TNF) inhibitors, the overall cancer RR was 1.40 (95% CI 1.06 to 2.08;  p= 0.02), indicating a modestly higher risk. This pattern was similar when examining all cancers except non-melanoma skin cancer (RR 1.47; 95% CI 1.05 to 2.06; p = 0.03). For non-melanoma skin cancer alone, no significant difference was observed (RR  1.30; 95% CI, 0.22–5.83; p= 0.88). In conclusion, the meta-analysis found no significant difference in overall cancer risk between tofacitinib and either placebo or biological drugs, while a slightly increased risk was observed compared with TNF inhibitors. The authors emphasized that further studies are required to more precisely define the cancer risk associated with tofacitinib therapy. [2]

A final meta-analysis, published in 2020, evaluated the risk of malignancy in patients with rheumatoid arthritis (RA) treated with non-TNF inhibitor biologics or tofacitinib. Out of 2,819 identified articles, 10 studies were included, encompassing over 40,587 patients with more than 87,622 patient-years of exposure to non-TNFi biologics and 2,221 patients with over 4,506 patient-years of exposure to tofacitinib. Pooled analyses showed no increased risk of overall cancer or specific cancer types in RA patients treated with rituximab (pooled RR 0.87; 95% CI 0.74 to 1.03), tocilizumab (pooled RR  0.92; 95% CI 0.79 to 1.06), or tofacitinib, compared with patients receiving csDMARDs or TNFi. In contrast, abatacept was associated with a slightly increased overall cancer risk (pooled RR = 1.13; 95% CI 1.02 to 1.24) and an increased risk of non-melanoma skin cancer (pooled RR  1.26; 95% CI 1.09 to 1.45) relative to csDMARDs or TNFi. Based on these findings, the authors concluded that among RA patients, a small but statistically significant increase in cancer risk was observed with abatacept exposure, while no increased risk was detected for rituximab, tocilizumab, or tofacitinib compared with csDMARDs or TNFi. [3]

A 2022 letter to the editor discussed findings from the ORAL Surveillance safety trial, which evaluated cardiovascular and cancer risks associated with tofacitinib in patients with RA. The authors noted that patients treated with tofacitinib had a higher incidence of cancer compared to those treated with TNF inhibitors, with a reported hazard ratio of 1.48 (95% CI 1.04 to 2.09). The trial was initially justified due to prior observations of increased cancer incidence, including lymphoma, in patients treated with tofacitinib. The letter highlighted that while the study reported overall cancer and non-melanoma skin cancer incidence, it did not provide lymphoma-specific rates, which are clinically relevant given that RA itself is associated with a 1.5- to 4-fold increased risk of lymphoma compared to the general population, primarily due to chronic inflammation and lymphocyte stimulation. The letter emphasized the importance of understanding lymphoma risk for treatment decisions, not only in RA but also in ulcerative colitis, where tofacitinib is commonly used. The letter concluded by advocating for greater transparency in informed consent and reporting of specific cancer outcomes, particularly lymphoma, in safety trials. In their reply to the letter, the ORAL Surveillance investigators clarified that all patients provided informed consent detailing the known risks of tofacitinib and TNF inhibitors, with updates following the dose reduction from 10 mg to 5 mg twice daily for safety reasons. They reported higher lymphoma incidence per 100 patient-years with tofacitinib (0.07 for 5 mg, 0.11 for 10 mg) compared with TNF inhibitors (0.02) and noted that differences in pathogenesis and patient characteristics limit extrapolation to ulcerative colitis. [4]

Additional evidence suggests that the risk of cancer with TNF inhibitors varies by the reporting study or review article. In a 2019 meta-analysis of 9 studies (N= 11,679 patients), the overall risk of new cancer or cancer recurrence with TNF inhibitors was 3.2/100 patient-years which was not significantly different compared to control therapies. A nationwide cohort study suggests that TNF inhibitors are associated with a lower risk of cancer compared to non-biologic disease-modifying anti-rheumatic drugs (nbDMARD) in a Korean population (incidence rate 6.5 vs 15.6 per 1000 patient-years). A 2021 systematic review specific to inflammatory disease patients found that among 28 included studies (N= 298,717 patients), the overall occurrence rate of cancer was 1.0%, but no significant association was found between TNF inhibitors and cancer in 10 of 11 studies. [5], [6], [7]

References:

[1] Solitano V, Ahuja D, Lee HH, et al. Comparative Safety of JAK Inhibitors vs TNF Antagonists in Immune-Mediated Inflammatory Diseases: A Systematic Review and Meta-Analysis. JAMA Netw Open. 2025;8(9):e2531204. Published 2025 Sep 2. doi:10.1001/jamanetworkopen.2025.31204
[2] Bezzio C, Vernero M, Ribaldone DG, Alimenti E, Manes G, Saibeni S. Cancer Risk in Patients Treated with the JAK Inhibitor Tofacitinib: Systematic Review and Meta-Analysis. Cancers (Basel). 2023;15(8):2197. Published 2023 Apr 7. doi:10.3390/cancers15082197
[3] Xie W, Yang X, Huang H, Gao D, Ji L, Zhang Z. Risk of malignancy with non-TNFi biologic or tofacitinib therapy in rheumatoid arthritis: A meta-analysis of observational studies. Semin Arthritis Rheum. 2020;50(5):930-937. doi:10.1016/j.semarthrit.2020.08.007
[4] Ytterberg SR, Bhatt DL, Mikuls TR, et al. Cardiovascular and Cancer Risk with Tofacitinib in Rheumatoid Arthritis. N Engl J Med. 2022;386(4):316-326. doi:10.1056/NEJMoa2109927
[5] Micic D, Komaki Y, Alavanja A, Rubin DT, Sakuraba A. Risk of Cancer Recurrence Among Individuals Exposed to Antitumor Necrosis Factor Therapy: A Systematic Review and Meta-Analysis of Observational Studies. J Clin Gastroenterol. 2019;53(1):e1-e11. doi:10.1097/MCG.0000000000000865
[6] Choi B, Park HJ, Song YK, Oh YJ, Kim IW, Oh JM. The risk of newly diagnosed cancer in patients with rheumatoid arthritis by TNF inhibitor use: a nationwide cohort study [published correction appears in Arthritis Res Ther. 2022 Oct 25;24(1):238]. Arthritis Res Ther. 2022;24(1):191. Published 2022 Aug 9. doi:10.1186/s13075-022-02868-w
[7] Muller M, D'Amico F, Bonovas S, Danese S, Peyrin-Biroulet L. TNF Inhibitors and Risk of Malignancy in Patients with Inflammatory Bowel Diseases: A Systematic Review. J Crohns Colitis. 2021;15(5):840-859. doi:10.1093/ecco-jcc/jjaa186

Literature Review

A search of the published medical literature revealed 3 studies investigating the researchable question:

What is the malignancy risk in patients with rheumatologic disease being treated with either a TNF inhibitor (such as adalimumab), JAK inhibitor (such as tofacitinib), or IL-6 inhibitor (such as tocilizumab)?

Level of evidence

C - Multiple studies with limitations or conflicting results  Read more→



Please see Tables 1-3 for your response.


 

Cancer risk in patients with rheumatoid arthritis receiving biologic and targeted synthetic disease-modifying antirheumatic drugs: results from the BIOBADASER III registry
Design

Multicentre observational, prospective cohort study

N= 4635

Objective To assess the risk of cancer in patients with rheumatoid arthritis (RA) treated with biologic and targeted synthetic DMARDs (b/tsDMARDs)
Study Groups

TNFis (n= 3034)

IL6is (n= 1106)

CD20i (n= 476)

JAKi (n= 1320)

CTLA4-A (n= 906)

Inclusion Criteria Patients diagnosed with RA aged 18 years or older treated with bDMARDs or tsDMARDs from January 2000 to December 2023 in BIOBADASER III
Exclusion Criteria Patients with a history of cancer
Methods Data from the BIOBADASER III registry were analyzed. Incidence rates (IRs) were calculated, and adjusted Cox regression models were used to estimate hazard ratios (HRs) for all cancers excluding non-melanoma skin cancer (NMSC), as well as for NMSC. Treatment with a TNF inhibitor (TNFi) served as the reference for comparison. 
Duration January 2000 to October 2023
Outcome Measures Occurrence of a first primary cancer during follow-up, cancer types including malignancy
Baseline Characteristics   TNFi (n= 3034) IL6i (n= 1106) CD20i (n= 476) JAKi (n= 1320) CTLA4-A (n= 906)
Female 2410 (79.4%) 905 (81.8%) 377 (79.2%) 1079 (81.7%) 678 (74.8%)
Age at treatment initiation 54.3 ± 12.3 56.9 ± 12.4 57.2 ± 11.6 56.0 ± 12.0 60.8 ± 12.1
Disease duration 5.7 (2.2–11.3) 9.1 (4.3–15.4) 10.7 (5.6–16.9) 9.5 (4.0–16.0) 9.5 (4.7–16.7)
RF positive 1727 (56.9%) 548 (49.6%) 209 (43.9%) 661 (50.1%) 495 (54.6%)
ACPA positive 1627 (53.6%) 503 (45.5%) 192 (40.3%) 641 (48.6%) 476 (52.5%)
Results Event TNFi IL6i CD20i JAKi CTLA4-A
All cancers excluding NMSC 88 41 15 31 34
NMSC 35 12 5 10 14
Melanoma 5 0 0 0 2
Solid cancer 75 38 13 28 34
Haematologic cancer 8 3 2 3 2
Lung cancer 15 6 2 5 12
Breast cancer 12 3 0 4 2
For all cancers excluding NMSC, the adjusted HRs (95% CI) compared with TNFi were 1.2 (0.8–1.6) for IL6i, 0.9 (0.5–1.4) for CD20i, 1.2 (0.8–1.8) for JAKi, and 1.1 (0.8–1.6) for CTLA4-A. For NMSC, the adjusted HRs (95% CI) were 0.6 (0.2–1.5) for IL6i, 0.6 (0.2–1.8) for CD20i, 0.7 (0.2–2) for JAKi, and 1.1 (0.5–2.6) for CTLA4-A. No increased cancer risk was seen after adjusting for cardiovascular risk or treatment line for either cancer type.
Adverse Events No increased cancer risk was observed when adjusting for cardiovascular risk or treatment line, for either cancer type. 
Study Author Conclusions In this large, real-world cohort of RA patients, we found no increased cancer risk associated with any bDMARDs or tsDMARDs compared with TNFi. 
Critique The study's strength lies in its large sample size and real-world data from a national registry, providing comprehensive insights into cancer risk associated with bDMARDs and tsDMARDs. However, the observational nature may introduce indication bias, and the exclusion of b/tsDMARD-naive patients limits comparisons. Additionally, the relatively low incidence of certain cancer types reduces the statistical power for some subanalyses. 
References:

Molina-Collada J, Otero-Varela L, Vela P, et al. Cancer risk in patients with rheumatoid arthritis receiving biologic and targeted synthetic disease modifying antirheumatic drugs: results from the BIOBADASER III registry. Rheumatology (Oxford). Published online October 22, 2025. doi:10.1093/rheumatology/keaf556

 

Comparison of risks of cancer, infection, and MACEs associated with JAK inhibitor and TNF inhibitor treatment: a multicentre cohort study
Design

Multicentre cohort study

N= 499

Objective To compare the incidence rates of infectious diseases, major adverse cardiovascular events (MACEs), and malignancies in RA patients treated with tofacitinib, baricitinib, or a TNF inhibitor
Study Groups

Tofacitinib (n= 192)

Baricitinib (n= 104)

TNF inhibitor (n= 203)

Inclusion Criteria Patients diagnosed with RA and treated with tofacitinib, baricitinib, or TNF inhibitors at Nagasaki University Hospital, Sasebo Chuo Hospital, or Ureshino Medical Center from March 2013 through December 2020
Exclusion Criteria Patients who declined to provide informed consent
Methods Retrospective analysis of 499 RA patients treated with tofacitinib, baricitinib, or TNF inhibitors. Incidence rates of infectious diseases and standardized incidence ratio of malignancies were determined. Propensity score weighting was used to adjust for clinical characteristic imbalances. JAK inhibitor-treated patients received tofacitinib 5 mg twice or once daily or baricitinib 2 mg or 4 mg once daily. 
Duration March 2013 through December 2020
Outcome Measures Incidence rates of infectious diseases, MACEs, and malignancies
Baseline Characteristics   Tofacitinib (n= 192) Baricitinib (n= 104) TNFi (n= 203)
Male:Female 37:155 2:11 37:166
Age, yrs 67 (58–73) 68 (58–75) 51 (37–61)
Disease duration, yrs 11 (6–18) 12 (3–18) 6 (2–14)
RF positivity 148 (77.1%) 81 (77.9%) 163/202 (80.7%)
ACPA positivity 144/185 (77.8%) 78 (78.8%) 150/184 (81.5%)
Concomitant glucocorticoid use  105 (54.7%) 42 (40.4%) 112 (55.2%)
Concomitant MTX use 135 (70.3%) 46 (44.2%) 132 (65.0%)
Results   JAKi Tofacitinib Baricitinib TNFi
Serious infectious diseases other than herpes zoster (HZ) 40 (13.5%) 33 (17.2%) 7 (6.7%) 16 (7.9%)
Serious infectious diseases other than HZ, IR per 100 PY (95% CI) 8.36 (7.79, 8.93) 8.68 (8.10, 9.26) 7.09 (6.57, 7.61) 4.09 (3.70–4.49)
HZ, No. 57 (19.3%) 46 (24.0%) 11 (10.6%) 6 (3.0%)
HZ, IR per 100 PY (95% CI) 13.00 (12.29, 13.71) 13.37 (12.65, 14.09) 11.63 (10.96, 12.30) 1.48 (1.24–1.72)
Compared with the general Japanese population, overall malignancy rates were similar between JAK-inhibitor and TNF-inhibitor treatments, with standardized incidence ratio (SIRs) of 1.61 (95% CI: 0.80–2.88) and 0.71 (95% CI: 0.15–2.07), respectively. Among JAK-inhibitor users, SIRs were 1.95 (95% CI: 0.93–3.58) in women and 0.59 (95% CI: 0.01–3.28) in men, and were comparable for tofacitinib (1.59, 95% CI: 0.73–3.02) and baricitinib (1.71, 95% CI: 0.21–6.16). Lung cancer was the most frequent malignancy, though its SIR with JAK-inhibitor treatment (2.19, 95% CI: 0.27–7.91) was not significantly elevated. Overall, there was a nonsignificant trend toward higher malignancy incidence with JAK inhibitors.
Adverse Events The IR of HZ in the JAK-inhibitor–treated group was significantly higher than the TNF-inhibitor-treated group. No significant differences in the IRs of other adverse events between the JAK-inhibitor–treated group and the TNF-inhibitor–treated group. 
Study Author Conclusions The infectious disease IR in RA was comparable between tofacitinib and baricitinib, but the IR for HZ in these treatment groups was high compared with that in the TNF inhibitor treatment group. The malignancy rate in the JAK-inhibitor-treated group was high but not significantly different from that of the general population or that of the TNF-inhibitor–treated group. 
Critique The study provides valuable real-world evidence on the safety of JAK inhibitors in RA treatment. However, the retrospective design and relatively short follow-up period for baricitinib may limit the ability to detect all adverse events. Larger studies with longer follow-up are needed to confirm these findings. 
References:

Uchida T, Iwamoto N, Fukui S, et al. Comparison of risks of cancer, infection, and MACEs associated with JAK inhibitor and TNF inhibitor treatment: a multicentre cohort study. Rheumatology (Oxford). 2023;62(10):3358-3365. doi:10.1093/rheumatology/kead079

Safety of JAK and IL-6 Inhibitors in Patients with Rheumatoid Arthritis: A Multicenter Cohort Study
Design

Multicenter, propensity-matched, retrospective cohort study

N= 220 (propensity-matched)

Objective To determine and compare the incidence rates of malignancies and major adverse cardiovascular events (MACEs) in patients with rheumatoid arthritis (RA) treated with an interleukin-6 inhibitor (IL-6i) or Janus kinase inhibitor (JAKi)
Study Groups

IL-6i (n= 110)

JAKi (n= 110)

Inclusion Criteria Patients diagnosed with RA (according to the 2010 ACR/EULAR classification criteria) treated with IL-6i or JAKi
Exclusion Criteria Patients who declined to provide informed consent
Methods A retrospective analysis of demographic and clinical data from patients treated for RA at three different hospitals between April 2012 and December 2022 was conducted. Patients were treated with either an IL-6i or a JAKi. Incidence rates of malignancy and MACEs were determined. Propensity score matching was used to adjust for imbalances in clinical characteristics. IL-6i treatments included tocilizumab and sarilumab. JAKi treatments included baricitinib, tofacitinib, upadacitinib, and filgotinib.
Duration April 2012 to December 2022
Outcome Measures

Primary: Incidence rates of malignancy and MACEs

Secondary: Standardized incidence ratio (SIR) of malignancies

Baseline Characteristics   IL-6i (n= 110) JAKi (n= 110) p-Value
Age at b/ts DMARD, median (range), years 67 (59.3–74) 68 (60–74) 0.436
Female 75 (68.2%) 75 (68.2%) 1
Disease duration, median (range), years 8.5 (3.7–14.0) 7.1 (2.1–15.7) 0.359
RF positivity 79 (71.8%) 74 (67.3%) 0.099
ACPA positivity 84 (76.4%) 78 (70.9%) 0.095
Concomitant GC use 29 (26.4%) 35 (31.8%) 0.120
Concomitant MTX use 53 (48.2%) 58 (52.7%) 0.091
Coexisting ILD 14 (12.7%) 14 (12.7%) <0.001
Coexisting hypertension 33 (30.0%) 28 (25.5%) 0.102
Coexisting DM 12 (10.9%) 13 (11.8%) 0.029
Previous history of malignancy 9 (8.2%) 9 (8.2%) <0.001
Previous use of b/ts DMARDs 40 (36.4%) 57 (51.8%) 0.315
Observation period, median (range), years 2.7 (1.2–4.5) 1.7 (1.0–3.3) 0.516
Abbreviations: ACPA= anti-citrullinated peptide antibody. b/ts DMARD= biologic/targeted synthetic disease-modifying antirheumatic drug. DM= diabetes mellitus. GC= glucocorticoid. IL-6i= interleukin-6 receptor inhibitors. ILD= interstitial lung disease. JAKi= Janus kinase inhibitors. MTX= methotrexate. RF= rheumatoid factor.
Results   IL-6i (n= 110) JAKi (n= 110) p-Value
Malignancy 5 (4.5%) 7 (6.4%) -
IR per 100 PY (95% CI) 1.36 (0.18–2.54) 2.94 (0.80–5.08) -
IRR (95% CI) 1 [reference] 2.13 (0.67-7.42) 0.20
MACEs 3 (2.7%) 6 (5.5%) -
IR per 100 PY (95% CI) 0.83 (–0.1–1.76) 2.56 (0.54–4.58) -
IRR (95% CI) 1 [reference] 3.03 (0.77–15.21) 0.11
Abbreviations: CI= confidence interval. IR= incidence rate. IRR= incidence rate ratio.  MACEs= major adverse cardiovascular events. PY= patient-years.
Adverse Events The incidence rate of malignancy was higher in the JAKi-treated group compared to the IL-6i-treated group. The incidence rate of MACEs was also higher in the JAKi-treated group. However, there were no significant differences in the incidence rate ratios for malignancy and MACEs between the groups after propensity score matching.
Study Author Conclusions The IRs of malignancy and MACE in patients with RA after propensity score matching were comparable between IL-6i-treated and JAKi-treated patients. However, the SIR of malignancy in JAKi treatment was significantly higher than in the general population; therefore, further safety studies comparing JAKi to non-TNFi biologic disease-modifying antirheumatic drugs (bDMARDs) are needed.
Critique The study provides valuable insights into the safety of JAK and IL-6 inhibitors in real-world settings, highlighting the importance of monitoring malignancy and MACE risks. However, the retrospective design, limited follow-up period, and potential confounding factors may limit the generalizability of the findings. The study's reliance on propensity score matching helps address some imbalances, but residual confounding cannot be ruled out. Further prospective studies with longer follow-up are needed to confirm these findings.
References:

Yoshida S, Miyata M, Suzuki E, et al. Safety of JAK and IL-6 inhibitors in patients with rheumatoid arthritis: a multicenter cohort study. Front Immunol. 2023;14:1267749. Published 2023 Oct 2. doi:10.3389/fimmu.2023.1267749