What is the newest evidence on fluconazole 150mg in pregnancy?

Comment by InpharmD Researcher

The most recent evidence regarding the use of fluconazole in pregnancy suggests use may be associated with malformations, teratogenic potential, and other adverse fetal outcomes. While higher doses (≥150 mg) may carry greater risk, pooled data from the past several years has found maternal exposure to fluconazole to be associated with increased risk of heart defects, cleft palate, and miscarriage. Although some of the supporting data are of low-quality due to the lack of adequate and well-controlled clinical trials, caution may be warranted in this setting.

Background

A 2021 review article examines the use of common antifungal drugs during pregnancy. Many pregnant women with fungal infections may require systemic therapy; however, oral antifungals such as fluconazole, itraconazole, and griseofulvin have been linked to birth defects and spontaneous abortions in some reports. Animal studies indicate potential fetal risk, and controlled studies in pregnant women are limited or lacking. The authors note that evidence suggests low-dose fluconazole (150 mg/day) may carry some fetal risk, but in specific situations, such as life-threatening infections or when alternative treatments are ineffective, the benefits may outweigh the risks. Higher doses of fluconazole (400-600 mg/day) appear to carry a greater risk of adverse fetal outcomes. Systemic azoles, including fluconazole and itraconazole, are fungistatic drugs shown to be teratogenic and embryotoxic in animal models, causing craniofacial and rib abnormalities. Observational studies in humans suggest that fluconazole exposure during pregnancy may be associated with spontaneous abortions, congenital heart defects (including cardiac septal defects), and musculoskeletal malformations such as abnormal skull development, oral clefts, bowed long bones, thin ribs, muscle weakness, and joint deformities. Population-based studies reinforce these findings. One cohort of nearly 2 million pregnancies found that first-trimester oral fluconazole exposure was associated with a modestly increased risk of musculoskeletal malformations compared with topical azoles, with an adjusted relative risk of 1.30 and an absolute risk increase of about 12 per 10,000 pregnancies. Other studies suggest potential links to cardiac malformations, including tetralogy of Fallot. Meta-analyses and systematic reviews indicate that these risks appear dose-dependent: higher doses (>150 mg) are linked to increased risk of cardiac defects, limb abnormalities, and spontaneous abortion, whereas lower doses (≤150 mg) appear relatively safer. [1]

A 2024 systematic review and meta-analysis investigated the potential risks associated with the use of oral fluconazole during the first trimester of pregnancy. The analysis included data from 9 observational studies, encompassing a total of 3,764,897 pregnancies with 116,425 women exposed to fluconazole to assess the association between fluconazole exposure and the occurrence of major congenital malformations (MCM) and miscarriages. Results highlighted a significant association between fluconazole exposure of any dose in the first trimester of pregnancy and overall MCM when considering crude odds ratios (ORc 1.18, 95% CI 1.08-1.29). However, this association was not apparent in analyses using adjusted odds ratios (ORa 1.02, 95% CI 0.98-1.07), suggesting that confounding factors may influence the outcomes. A significant crude association for risk of MCM was noted between doses ≤ 150 mg (ORc 1.12, 95% CI 1.04-1.19) and doses > 150 mg (ORc 1.22, 95% CI 1.06-1.40). Despite these findings, the certainty of evidence ranged from very low to low, underscoring the need for further research to confirm these associations and guide clinical decision-making. The study calls for standardized definitions and methodologies to improve the reliability and comparability of future investigations into the teratogenic risks of fluconazole.[2]

A 2021 meta-analysis evaluated the teratogenic potential of maternal fluconazole use during pregnancy by analyzing 9 studies encompassing 53,407 fluconazole-exposed pregnant women compared to 3,319,353 unexposed women. This meta-analysis aimed to provide further clarity regarding the association between fluconazole exposure during the first trimester and congenital malformations in offspring. Dosing was stratified based on low dose (≤ 150 mg), high dose (> 150 mg), or any exposure (any dose). Results indicated that maternal exposure to fluconazole was associated with an increased risk of heart defects in offspring for both low-dose (OR 1.95, 95% CI 1.18-3.21; p= 0.01) and any dose exposure (OR 1.79, 95% CI 1.18-2.71; p= 0.01). Notably, no significant difference was observed between high-dose fluconazole versus control (OR 3.13, 95% CI 0.53-18.61; p= 0.21). However, this comparison involved high-heterogeneity (I2= 97.7%) and more limited data from 3 studies. The meta-analysis also explored other types of malformations and outcomes, revealing no significant associations between fluconazole exposure (low-dose, high-dose, or any exposure) and risks of spontaneous abortion, stillbirth, or other major congenital malformations such as orofacial, musculoskeletal, central nervous system, gastrointestinal, and genitourinary defects. Despite these findings, the substantial heterogeneity observed in heart defect analyses (I2 = 80.2% for low-dose and I2 = 82.8% for any-dose) underscores the complexity of fluconazole's teratogenic potential. [3]

Another 2020 systematic review and meta-analysis investigated fetal outcomes following maternal exposure to oral antifungal agents during pregnancy. The review included 8 cohort studies and one case-control study. The studies predominantly involved oral fluconazole and itraconazole used during pregnancy. In total, data were analyzed from 14,534 pregnancies exposed to fluconazole and 1,311 exposed to itraconazole, focusing on birth defects, spontaneous abortion, and stillbirth outcomes, and incidence rates were compared to values for the general population published by EUROCAT. Data from 4 case control studies revealed that oral fluconazole exposure during pregnancy may slightly increase the risk of congenital heart defects (frequency 1.52% vs. 0.77% for general population) and limb defects (0.62% vs. 0.56%). In contrast, oral itraconazole exposure might heighten the risk of eye defects. However, the study found no significant overall increase in the risk of other birth defects, spontaneous abortions, or stillbirths in comparison to non-exposed groups. One case control study compared fluconazole exposed women to control women, finding fluconazole exposure during the first trimester was significantly associated with cleft palate (OR, 5.53; 95% CI 1.68-18.24) and dextro-transposition of the great arteries (OR 7.56; 95% CI 1.22-35.45). Despite these findings, the authors suggest that the risks associated with fluconazole and itraconazole necessitate further cautious investigation, especially concerning specific birth defects. In this study, effects were not stratified based on fluconazole dose due to inadequate data. [4]

References:

[1] Patel MA, Aliporewala VM, Patel DA. Common Antifungal Drugs in Pregnancy: Risks and Precautions. J Obstet Gynaecol India. 2021;71(6):577-582. doi:10.1007/s13224-021-01586-8
[2] Latour M, Vauzelle C, Elefant E, et al. Risk of congenital malformations and miscarriages following maternal use of oral fluconazole during the first trimester of pregnancy: a systematic review and meta-analysis. Eur J Epidemiol. 2024;39(12):1325-1340. doi:10.1007/s10654-024-01177-7
[3] Budani MC, Fensore S, Di Marzio M, Tiboni GM. Maternal use of fluconazole and congenital malformations in the progeny: A meta-analysis of the literature. Reprod Toxicol. 2021;100:42-51. doi:10.1016/j.reprotox.2020.12.018
[4] Liu D, Zhang C, Wu L, Zhang L, Zhang L. Fetal outcomes after maternal exposure to oral antifungal agents during pregnancy: A systematic review and meta-analysis. Int J Gynaecol Obstet. 2020;148(1):6-13. doi:10.1002/ijgo.12993

Relevant Prescribing Information

Warnings and Precautions [5]
Potential for fetal harm There are no adequate and well-controlled clinical trials of fluconazole in pregnant women. Case reports describe a pattern of distinct congenital anomalies in infants exposed in utero to high-dose maternal fluconazole (400 to 800 mg/day) during most or all of the first trimester. These reported anomalies are similar to those seen in animal studies. If fluconazole is used during pregnancy or if the patient becomes pregnant while taking the drug, the patient should be informed of the potential hazard to the fetus. Effective contraceptive measures should be considered in women of child-bearing potential who are being treated with fluconazole 400 to 800 mg/day and should continue throughout the treatment period and for approximately 1 week (5 to 6 half-lives) after the final dose. Epidemiological studies suggest a potential risk of spontaneous abortion and congenital abnormalities in infants whose mothers were treated with 150 mg of fluconazole as a single or repeated dose in the first trimester, but these epidemiological studies have limitations and these findings have not been confirmed in controlled clinical trials.

References:

[5] Fluconazole oral tablet. Prescribing information. Dr. Reddy's Laboratories Limited; 2025.

Literature Review

A search of the published medical literature revealed 2 studies investigating the researchable question:

What is the newest evidence on fluconazole 150mg in pregnancy?

Level of evidence

C - Multiple studies with limitations or conflicting results  Read more→



Please see Tables 1-2 for your response.


Exposure to Systemic Antimicrobials During Pregnancy and Risk of Miscarriage: A Population-Based Registry Study
Design

Population-based cohort study

N= 704,082

Objective To estimate miscarriage risk following gestational antimicrobial exposure while addressing biases that have affected previous studies
Study Groups

Exposed to systemic antimicrobials (n= 91,836)

Unexposed (n= 612,246)

Inclusion Criteria Pregnancies from 2009 to 2018, with exposure to systemic antimicrobials in early pregnancy
Exclusion Criteria Ectopic and molar pregnancies, miscarriages before 14 gestational days, pregnancies exposed to teratogenic medications
Methods Time-stratified Cox regression models with overlap weights were used, considering time-varying exposures and a 14-day lag to prevent reverse causation. Elective terminations were right-censored to address competing risks, with adjustment for common infections and probabilistic bias analysis for confounding by indication. 
Duration 2009 to 2018
Outcome Measures Associations of specific antimicrobials with miscarriage risk
Baseline Characteristics   Exposed (n= 91,836) Unexposed (n= 612,246) Overall (n= 704,082)

Start of last menstrual period (LMP)

2009–2011

 

33,475 (36.5%)

 

206,828 (33.8%)

 

240,303 (34.1%)

Maternal age, years

< 20

 

2,587 (2.8%)

 

11,248 (1.8%)

 

13,835 (2.0%)

Marital status  

Married/cohabiting

 

79,752 (86.8%)

 

541,099 (88.4%)

 

620,851 (88.2%)

Parity

Nulliparous

 

36,245 (39.5%)

 

235,644 (38.5%)

 

271,889 (38.6%)

History of miscarriage 24,759 (27.0%) 153,395 (25.0%) 178,154 (25.3%)
Smoking in pregnancy 9,541 (10.4%) 43,270 (7.1%) 52,811 (7.5%)
Folic acid use in pregnancy 68,928 (75.1%) 459,118 (75.0%) 528,046 (75.0%)

Infections during pregnancy

Respiratory tract infection

Urinary tract infection

 

22,457 (24.4%)

26,580 (28.9%)

 

31,282 (5.1%)

6,990 (1.1%)

 

53,739 (7.6%)

33,570 (4.8%)

Results   Hazard Ratio (95% CI)
Nitrofurantoin 0.75 (0.66–0.84)
Pivmecillinam 0.91 (0.87–0.95)
Amoxicillin 0.91 (0.87–1.05)
Metronidazole 2.00 (1.82–2.21)
Ciprofloxacin 1.89 (1.62–2.20)
Cephalexin 1.87 (1.57–2.22)
Fluconazole 1.61 (1.45–1.78)
Trimethoprim-sulfas 1.49 (1.36–1.63)
Adverse Events Not applicable
Study Author Conclusions Nitrofurantoin, pivmecillinam and amoxicillin did not increase miscarriage risk, but other less commonly used antimicrobials may carry higher risks. By addressing key biases, this study provided a more reliable assessment of miscarriage risks associated with antimicrobial use in early pregnancy. 
Critique The study's strengths include its large sample size and the use of multiple registries to address biases such as immortal time and reverse causation. However, limitations include potential residual confounding by socioeconomic status, BMI, and infection severity, as well as the inability to capture non-clinically recognized miscarriages and medications dispensed during hospital admissions. 
References:

Boissiere-O'Neill T, van Gelder MMHJ, Engjom HM, Nordeng HME. Exposure to Systemic Antimicrobials During Pregnancy and Risk of Miscarriage: A Population-Based Registry Study. BJOG. Published online March 28, 2025. doi:10.1111/1471-0528.18155

 

Common teratogenic medication exposures—a population-based study of pregnancies in the United States
Design

Cross-sectional study

N=2,223,749

Objective To identify medications with known or potential teratogenic risk commonly used during pregnancy among privately insured persons
Study Groups

Live-birth pregnancies (N=1,634,457)

Non-live-birth pregnancies (N=589,292)

Inclusion Criteria Persons aged 12 to 55 years with continuous health plan enrollment from 90 days before conception until 30 days after the pregnancy end date
Exclusion Criteria Sex hormones and hormone analogs, abortion and postpartum/abortion hemorrhage treatments, opioids, and anti-obesity medications if their only teratogenic mechanism was weight loss
Methods Used the MerativeTM MarketScan Commercial Database to identify pregnancies and medication exposures. Medications with known or potential teratogenic risk were selected from TERIS and drug monographs. Prenatal exposure was defined based on outpatient pharmacy claims or medical encounters during target pregnancy periods. 
Duration 2011 to 2018
Outcome Measures Prevalence of prenatal exposure to medications with known or potential teratogenic risk Secular trends in prenatal exposure
Baseline Characteristics   All pregnancies (N=2,223,749) Live-birth pregnancies (N=1,634,457) Non-live-birth pregnancies (N=589,292)

Age

< 20 years

20 - 24 years

25 - 29 years

30 - 34 years

35 - 39 years

40 - 44 years

>= 45 years

 

2.99%

12.58%

24.33%

34.11%

19.62%

5.51%

0.85%

 

2.63%

12.21%

25.51%

36.05%

18.90%

4.06%

0.63%

 

3.98%

13.63%

21.05%

28.73%

21.61%

9.53%

1.47%

Region

Northeast

North Central

South

West

Unknown

 

18.38%

21.30%

39.64%

19.65%

1.03%

 

16.70%

22.01%

40.78%

19.51%

1.00%

 

23.05%

19.32%

36.50%

20.03%

1.11%

Results Rate per 100,000 pregnancy year (PY) All pregnancies Live-birth pregnancies Non-live-birth pregnancies
Sulfamethoxazole/trimethoprim 1988  1740.1  4838.3 
High-dose fluconazole 1247.8  1206.7  1720.1 
Topiramate 351.2  238.8  1643.8 
Lisinopril 143.9  140.6  594.8 
Warfarin 57.0  31.3  352.0 
Losartan 56.3  55.2  203.9 
Carbamazepine 50.3 40.5  163.5 
Valproate 48.8  31.9  243.7
Vedolizumab 28.1  20.2  124.6 
Valsartan 25.3  24.6  119.0 
Top 10 medications with potential teratogenic risk
Fluconazole (low-dose) 6494.7 6108.1 10,937.3
Metoprolol 1324.7 1149.8 1959.1
Atenolol 447.8 369.4 732.3
Duloxetine 421.9 301.1 1810.3
Gabapentin 371.7  268.4 1558.6
Lidocaine 317.4 308.5 1546.4
Methimazole 132.4 120.4 269.8
Hydralazine 122.4 116.9 866.7
Lithium 121.8 88.7 242.0
Meloxicam 106.0 104.9 254.9
Adverse Events See results
Study Author Conclusions Several medications with teratogenic risk continue to be used during pregnancy despite the availability of safer alternatives. Regular reevaluation of risk mitigation strategies is needed to reduce exposures. 
Critique The study provides a comprehensive overview of teratogenic medication exposures using a large dataset, which is a strength. However, it relies on claims data, which may not accurately reflect actual medication consumption. The study is limited to privately insured populations, which may not be generalizable to the entire US population. Additionally, the study does not explore the clinical context of medication use, which is crucial for understanding the reasons behind these exposures. 
References:

Wang Y, Smolinski NE, Thai TN, et al. Common teratogenic medication exposures-a population-based study of pregnancies in the United States. Am J Obstet Gynecol MFM. 2024;6(1):101245. doi:10.1016/j.ajogmf.2023.101245