Is it safe to use alteplase for acute ischemic stroke in patients who are taking direct oral anticoagulants (DOACs)?

Comment by InpharmD Researcher

There is limited data on the safety of thrombolytic therapy in patients taking direct oral anticoagulants (DOACs). Studies have reported that the use of DOACs has not been associated with a significant increase in the risk of bleeding following thrombolytic therapy (e.g., intravenous alteplase). However, most data come from observational studies, which are considered low-quality evidence due to the nature of the study design. Clinical guidelines recommend that patients taking DOACs may be eligible for intravenous alteplase if it has been at least 48 hours since the last dose of DOAC and/or anticoagulation laboratory are normal. Results from a retrospective study support the guidelines’ recommendation of a 48-hour timeline; however, the study has a small size. Finally, although it is recommended to monitor anticoagulation laboratory values, evidence suggests not all commonly used anticoagulation lab values can reliably quantify the effect of DOACs.

  

A comprehensive literature search was conducted in PubMed using the following key terms: alteplase, direct oral anticoagulants, thrombolytic agents, intracranial hemorrhage. The search was expanded to include non-vitamin k oral antagonists, acute ischemic stroke, and atrial fibrillation.

Background

According to the American Heart Association (AHA) guidelines, the safety of intravenous (IV) alteplase in patients on direct oral anticoagulants (DOACs) remains debatable. Moreover, IV alteplase should not be considered in such population unless all laboratory values (aPTT, INR, thrombin time, platelet count, ecarin clotting time, or other appropriate factor Xa assays) return normal, or it has been > 48 hours since the last dose of DOAC administration (assuming normal renal function). This recommendation is expert consensus based on clinical experiences. [1]

It is stated that, although the guidelines recommend using aPTT, INR, TT, PT, and/or ECT to monitor the effect of anticoagulation to determine the eligibility for alteplase, these measurements are qualitative but are often inadequate in reliably quantifying the effect of anticoagulation of non-vitamin K oral anticoagulants (NOACs; e.g., dabigatran, apixaban, rivaroxaban). Moreover, there is no clinical data available regarding when is the optimal time to restart NOACs after acute ischemic stroke. [2]

In a review of 55 studies, 492 patients NOACs (dabigatran, apixaban, rivaroxaban, non-specified NOAC) who received IV tPA for acute stroke were identified. The included population was elderly (median age 77; interquartile range [IQR] 68-83) with median National Institutes of Health Stroke Scale score of 10 (IQR 6-16). The median time from last NOAC intake to stroke onset was 8 hours (IQR 2.5-14.5). The majority of the patients took NOAC within 12 hours of stroke onset (55.2%; 80/145). About a third took NOAC 13-24 hours (33.7%; 43/127), and 7.7% (10/130) took >24 hours preceding stroke onset. aPTT was the most commonly used coagulation test, and 27.4% (34/124) had prolonged aPTT levels. Other reported lab data are prolonged PT (7/16; 43.8%); prolonged TT in dabigatran (23/27 85.2%); TT, dTT, dabigatran concentration in dabigatran (37/94; 39.4%); and anti-Xa assays in rivaroxaban or apixaban (43/91; 47.3%). Of note, baseline characteristics information was not available for all patients. For clinical outcomes, the rate of sICH was reported in 4.3% (20/462), death was reported in 11.3% (48/423), and favorable outcomes were reported in 43.7% (164/375) patients. Favorable outcomes are defined as NIHSS, ≤1; mRS, 0–2; or improvement in NIHSS score, ≥8 points. Among those taking dabigatran, reversal with idarucizumab was associated with more favorable outcomes (79.1% [34/43] vs. 39.2% [29/74]; unadjusted OR 5.86; 95% CI, 2.45–14.00). There was no significant difference in the rate of sICH (unadjusted OR 0.60; 95% CI 0.12-2.92) or death (unadjusted OR 0.35; 95% CI 0.08-1.61). The authors conclude that IV tPA for acute ischemic stroke in patients receiving NOAC appears to be feasible without significant bleeding risks. There are several limitations to this review. First, the data were collected from case reports or case series, and some data were incomplete (e.g., baseline characteristics). There was no control group and pooled analysis was not performed; therefore, risk assessment data are not available. Finally, the study included patients on various NOAC, but the effects of individual NOACs were not reported/compared to each other. [3]

In a meta-analysis that evaluated data from 52,823 stroke patients, 366 patients were taking DOAC (warfarin: n= 2,133; no previous anticoagulation: n= 50,324). The study reported that, compared to warfarin, receiving a DOAC during the last 48 hours before bolus tPA was not associated with an increased risk of symptomatic intracranial hemorrhage (sICH) after intravenous thrombolysis (IVT) treatment [Odds ratio (OR) sICH according to NINDS definition: 0.53 [95% CI, 0.18–1.52]; OR for sICH according to ECASS-II definition: 0.77 [95% CI, 0.28–2.16]. In addition, the risk of sICH did not differ when compared to those with no history of anticoagulation (OR for sICH according to NINDS definition: 1.23 [95% CI, 0.46–3.31]; OR for sICH according to ECASS-II definition: 0.92 [95% CI, 0.33–2.55]). The results did not change when analyses were performed with no time limit. Compared to warfarin, the risk of sICH in those pretreated with a DOAC before tPA was OR: 0.85 [95% CI, 0.49–1.45] sICH according to NINDS definition; ECASS-II definition: OR 1.11 [95% CI, 0.67–1.85]. Compared to no previous anticoagulation, the risks were OR 1.17 [95% CI, 0.43–3.15] sICH according to NINDS definition; ECASS-II definition: 0.87 [95% CI, 0.33–2.41]]. For all data, there were no significant concerns for data heterogeneity (I2 = 0). [4]

References:

[1] Powers WJ, Rabinstein AA, Ackerson T, et al. Guidelines for the early management of patients with acute ischemic stroke: 2019 Update to the 2018 guidelines for the early management of acute ischemic stroke: A guideline for healthcare professionals from the American Heart Association/American Stroke Association. J. Am. Heart Assoc. 2019;50(12):344-418.
[2] Hankey GJ, Norrving B, Hacke W, Steiner T. Management of acute stroke in patients taking novel oral anticoagulants. Int J Stroke. 2014;9(5):627-632.
[3] Jin C, Huang RJ, Peterson ED, Laskowitz DT, et al. Intravenous tPA (tissue-type plasminogen activator) in patients with acute ischemic stroke taking non-vitamin k antagonist oral anticoagulants preceding stroke. J. Am. Heart Assoc. 2018;49(9):2237-2240
[4] Shahjouei S, Tsivgoulis G, Goyal N, et al. Safety of intravenous thrombolysis among patients taking direct oral anticoagulants: A systematic review and meta-analysis. J. Am. Heart Assoc 2020;51(2):533-541.

Literature Review

A search of the published medical literature revealed 3 studies investigating the researchable question:

Could you please summarize the available literature regarding the safety of administering alteplase (0.09 mg/kg IV bolus over 1 minute, followed by 0.81 mg/kg as a continuous infusion over 60 minutes, max total dose 90 mg) for acute ischemic stroke in the patient populations on DOACs (e.g. apixaban, rivaroxaban, edoxaban, and dabigatrain) at the time of presentation for the stroke event.

Please see Tables 1-3 for your response.


Use of Intravenous Recombinant Tissue Plasminogen Activator in Patients With Acute Ischemic Stroke Who Take Non–Vitamin K Antagonist Oral Anticoagulants Before Stroke

 

Design

Retrospective cohort study (N= 42,887)

Objective

To examine the outcomes of thrombolytic therapy in patients with ischemic stroke who received anticoagulation with non-vitamin K antagonist oral anticoagulants (NOACs) versus those on warfarin (international normalized ratio <1.7) or not on anticoagulation

Study Groups

NOACs (n= 251)

Warfarin (n= 1,500)

No anticoagulant (n= 41,136)

Methods

Data were collected from American Heart Association Get With The Guidelines-Stroke Registry (1,289 hospitals)

 

NOACs studied: dabigatran, rivaroxaban, apixaban, and edoxaban

 

Inclusion:

-       patients with acute ischemic stroke who have been treated with intravenous rt-PA only

Exclusion:

-       missing information of anticoagulant or antiplatelet therapy before a stroke

-       received rt-PA beyond the 4.5-hour treatment window or investigational or experimental protocol for thrombolysis

-       treated with heparin or anticoagulants rather than NOACs or warfarin before a stroke

 

Duration

October 2012 - March 2015

Outcome Measures

Primary outcomes:

-       Life-threatening or serious systemic hemorrhage within 36 hours

-       Any rt-PA complication within 36 hours (symptomatic intracranial hemorrhage, life-threatening or serious systemic hemorrhage<36 hours, or other serious complications).

-       symptomatic intracranial hemorrhage was defined as intracranial hemorrhage within a 36 hour

 

Secondary Outcomes: 

-       In-hospital mortality

-       Discharge destination (home, hospice, skilled nursing facility)

-       Ambulatory status

-       Modified Rankin Scale (mRS, a functional outcome measure ranging from 0 of no symptoms at all to 6 of death) at hospital discharge.

 

 

Baseline Characteristics

Variables

NOACs (n= 245)

Warfarin with INR<1.7 (n=245)

No oral anticoagulant (n= 245)

Age, years - median (range)

74 (66, 82)

75 (63, 85)

74 (62, 84)

Women - no. (%)

128 (52.2)

138 (56.3)

117 (47.8)

White

178 (72.7)

169 (69.0)

178 (72.7)

Medical History - no. (%)

-

-

-

Atrial Fibrillation

191 (78.0)

182 (74.3)

192 (78.4)

 Previous stroke/TIA

76 (31.0)

67 (27.3)

 76 (31.0)

CAD/MI

76 (31.0)

 58 (23.7)

62 (25.3)

Hypertension

193 (78.8)

195 (79.6)

184 (75.1)

Dyslipidemia

103 (42.0)

94 (38.4)

100 (40.8)

Diabetes mellitus

63 (25.7)

64 (26.1)

62 (25.3)

 

Results

Primary and secondary outcomes after propensity score matching

 

No. events (%)

 

 

 

NOACs (n=245)

Warfarin with INR<1.7 (n=245)

No Oral anticoagulant (n= 245)

Odds ratio NOACs vs No

(95% CI)

Odds ratio Warfarin vs No

(95% CI)

Symptomatic intracranial hemorrhage <36 hours

12/245(4.9)

12/245 (4.9)

15/245 (6.1)

0.79 (0.36–1.72)

0.79 (0.36–1.72)

Life threatening or serious systemic hemorrhage < 6 hours

1/245 (0.4)

1/245 (0.4)

1/245 (0.4)

1.00 (0.06–16.1)

1.00 (0.06–16.1)

Any alteplase complication

16/245 (6.5)

23/245 (9.4)

24/245 (9.8)

0.64 (0.33–1.24)

0.95 (0.53–1.74)

Secondary outcomes

Hospital mortality

12/245 (9.4)

21/245 (8.6)

24/245 (9.8)

0.95 (0.52–1.74

0.86 (0.47–1.60)

Discharge to home

101/245 (41.2)

88/245 (35.9)

86/245 (35.1)

1.30 (0.90–1.87)

1.04 (0.72–1.50)

Discharge to hospice

19/245 (7.8)

26/245 (10.6)

24/245 (9.8)

0.77 (0.41–1.45)

1.09 (0.61–1.96)

Discharge to nursing facility

62/245 (25.3)

61/245 (24.9)

45/245 (18.4)

0.72 (0.44–1.16)

0.95 (0.60–1.50)

Able to amputate independently at discharge

102/226 (45.1)

80/225 (35.6)

89/225(39.6)

1.26 (0.86–1.83)

0.84 (0.58–1.24)

Modified rankin scale at discharge

mRS 0-1

34/149 (22.8)

23/151 (15.2)

38/143(26.6)

0.82 (0.48–1.39)

0.50 (0.28–0.89)

mRS 0-2

44/149 (29.5)

44/151 (29.5)

47/143(32.9)

0.86 (0.52–1.41)

0.84 (0.51–1.38)

 

 

Adverse Events

Common Adverse Events: N/A

Serious Adverse Events: N/A

Percentage that Discontinued due to Adverse Events: N/A

Study Author Conclusions

The use of intravenous recombinant t-PA in patients taking NOAC was not associated with an increased risk of bleeding, mortality, or worse outcomes post-discharge.

InpharmD Researcher Critique

The authors discussed potential selection bias against NOAC patients treated with rtPA because patients on NOAC were more likely to be not treated with rtPA due to treatment avoidance in milder stroke severity and late arrival. The database used does not provide the time of the last dose taken; therefore, the relationship between bleeding risk of the timing of the last dose cannot be established. No baseline laboratory values were collected other than baseline INR. Given that INR is not an optimal assessment of anticoagulation effects of NOACs, patients’ baseline risks could not be assessed. Finally, the data is from the in-patient population and long-term outcomes in ambulatory/outpatient settings cannot be established.

 

References:

Xian Y, Federspiel JJ, Hernandez AF, et al., Use of intravenous recombinant tissue plasminogen activator in patients with acute ischemic stroke who take non-vitamin k antagonist oral anticoagulants before stroke. Circulation. 2017;135(11):1024-1035.

Recanalization therapies in acute ischemic stroke patients: impact of prior treatment with novel oral anticoagulants on bleeding complications and outcome

 

Design

Prospective cohort study (N= 9,457 total; n=612 propensity score-matched sample)

Objective

To investigate the safety of intravenous thrombolysis (IVT) or intra-arterial treatment (IAT) in patients taking non-vitamin K oral anticoagulants (NOACs)

Study Groups

NOACs (n= 78; propensity score-matched sample n= 63)

Warfarin (n= 441; propensity score-matched sample n= 339)

No anticoagulant (n= 8,938; propensity score-matched sample n= 210)

Methods

Data Collection:

-       National Institute of Health Stroke Scale (NIHSS) was used to evaluate patients before IVT/IVA and at 24 hours

-       Trial of Org 10172 in Acute Stroke Treatment (TOAST) criteria was used to assess occlusion, blood pressure, time-to-treatment, etiology

-       In patients taking NOAC, agent, daily dosage, the reason for usage, last intake  (in hours before IVT/IAT), and reason for using IVT/IAT treatment were recorded

-       Functional 3-month outcome was assessed using Rankin Scale (mRS) at outpatient visits or telephone interviews

 

NOACs studied: dabigatran, rivaroxaban, apixaban

Duration

Approval of the first NOAC for stroke prevention in AF in each country up to December 31, 2014

 

Outcome Measures

Primary outcomes:

-       The occurrence of intracranial hemorrhage in 3 categories, or death within 3 months

-       any ICH on follow-up imaging including hemorrhagic transformation

-       symptomatic ICH (sICH) according to the criteria of the European Cooperative Acute Stroke Study II (ECASS-II) trial (sICHECASS-II)

-       sICH based on the criteria of the National Institute of Neurological Disorders and Stroke (NINDS) thrombolysis trial (sICHNINDS)

 

Secondary Outcomes: 

-       National institutes health stroke scale (NIHSS) at 24 hours

-       Major neurological improvement, defined as an improvement in NIHSS score of 8 points at 24 hours in comparison with the initial NIHSS (or an NIHSS score of 0 at 24 hours) as defined in previous research

-       Favorable 3-month outcome (modified Rank Score of 0, 1, or 2)

 

 

Baseline Characteristics

Baseline characteristics after propensity score matching

Variables

NOACs (n= 63)

Vitamin K antagonist (n=339)

No oral anticoagulant (n= 210)

Age, years - median (range)

76 (64–81)

77 (68–83)

75 (67–81)

Women - no. (%)

28 (45)

158 (46.6)

108 (51.5)

Time-to-treatment, min - median (IQR)

176 (117–242)

141 (93–180)

132 (90–176)

NIHSS score, median (IQR)

15 (7–19)

14 (8–19)

14 (8–19)

INR, median (IQR)

1.14 (1.07–1.3)

1.3 (1.1–1.59)

1.05 (1–1.12)

 Type of acute recanalization therapy

IV thrombolysis only, n (%)

34 (53.5)

287 (84.8)

196 (93.2)

IV thrombolysis and IA treatment, n (%)

4 (6.3)

29 (8.7)

13 (6.1)

IA treatment only, n (%)

25 (40.2)

22 (6.5)

1 (0.7)

Concomitant treatment

Previous use of statins, n (%

25 (40)

112 (34.1)

71 (35.9)

Previous use of antihypertensive drugs, n (%)

56 (88.9)

275 (82.4)

180 (87.2)

Previous treatment with antiplatelets, n (%))

13 (19.9)

49 (14.6)

51 (24.5)

 

Results

Results after propensity score match

 

Novel Oral Anticoagulants (n=63)

Vitamin K Antagonists

(n=339)

no-OAC

(n=210)

p- value

Primary outcome - no. (%)

Any intracranial hemorrhage

13 of 62 (20.8%)

85 of 300 (28.4%)

44 of 184 (23.8%))

0.30

Symptomatic intracranial hemorrhage (ECASS-II)

2 of 62 (3.2%)

21 of 338 (6.1%)

14 of 207 (6.6%)

0.48

Symptomatic intracranial hemorrhage

(NINDS)

3 of 62 (4.8%

30 of 338 (8.9%)

19 of 203 (9.6%

0.56

Death at 3 months

16 of 60 (26.1%)

76 of 324 (23.6%)

43 of 204 (20.9%)

0.44

Secondary outcomes

NIHSS at 24 hours  - median (IQR)

10 (2–15)

 8 (3–16)

 8 (3–17)

0.45

Favorable clinical outcome at 3 mo (mRS0-2)

23 of 60 (39%)

138 of 324 (42.7%)

88 of 204 (43.2%)

0.03

 

Adverse Events

Common Adverse Events: N/A

Serious Adverse Events: N/A

Percentage that Discontinued due to Adverse Events: N/A

Study Author Conclusions

Use of NOAC is not associated with an increased risk of intracranial hematoma in IVT/IAT treatments; however, the following assumptions may need to be met: 1) treatments are provided in an experienced stroke center and 2) only select patients based on time since the last dose and baseline lab values are made eligible for IVT/IAT.

InpharmD Researcher Critique

Despite the relatively large total sample size, the sample size of NOAC is small. The study did not compare the bleeding risk between patients on NOAC treated IAT/IVT to that of patients on NOAC not treated with IAT/IVT.

References:

Seiffge DJ, Hooff RJ, Nolte CH, et al., Recanalization therapies in acute ischemic stroke patients: impact of prior treatment with novel oral anticoagulants on bleeding complications and outcome. Circulation. 2015;132(13):1261-1269.

Outcome of Patients Receiving Thrombolytic Therapy While on Rivaroxaban for Nonvalvular Atrial Fibrillation (from Rivaroxaban Once Daily Oral Direct Factor Xa Inhibition Compared With Vitamin K Antagonism for Prevention of Stroke and Embolism Trial in Atrial Fibrillation)

Design

Retrospective analysis of data collected from a randomized controlled trial (ROCKET AF; N= 28)

Objective

To analyze the outcomes of all patients who received thrombolytic therapy in the Rivaroxaban Once Daily Oral Direct Factor Xa Inhibition Compared With Vitamin K Antagonism for Prevention of Stroke and Embolism Trial in Atrial Fibrillation (ROCKET AF) trial

Study Groups

rivaroxaban (n= 19) 

warfarin (n= 9)

Methods

Patients enrolled in ROCKETAF trial who received thrombolytic therapy were identified, and their medical charts were reviewed.

 

Patients included in ROCKETAF had nonvalvular atrial fibrillation (AF) with moderate to high risk for stroke (CHADS2 at least 2)

 

Dose:

-       Rivaroxaban 20mg tablet daily

-       Dose-adjusted warfarin for an INR 2.5

Duration

Median follow-up time: 1.9 years

Outcome Measures

The safety outcomes

-       30-day post-thrombolytic rates of stroke bleeding, and mortality.

 

Baseline Characteristics

Persistent AF: 86%

Mean CHADS2: 35

Mean creatinine clearance 61 mL/min

Mean HAS-BLED: Rivaroxaban 3.11 (SD 0.74) vs. warfarin 3.0 (SD 0.87)

 

Warfarin (n= 9)

Rivaroxaban (n= 19)

Time since the last dose - no.

-

-

< 48 hours

5

11

48 - 1 week

1

4

> 1 week

3

3

Indication

-

-

 Ischemic stroke

1

9

Graft Occlusion

1

1

Myocardial infarction

1

1

Unknown

6

7

Thrombolytic agent

-

-

 Alteplase

5

9

Streptokinase

3

2

Reteplase

1

-

Urokinase

 

6

Fibrinolysin

 

2

 

Results

Post-thrombolysis 30 day outcomes

Total (N= 28)

Rivaroxaban (n= 19)

Warfarin (n= 9)

All events

9

5

4

< 48 hours from last study drug dose 

-

-

-

Ischemic stroke

1

1

0

Hemorrhagic conversion

1

1

0

Major NMCR bleeding

1

1

0

Mortality

2

1

1

48 hours to 1 week from last study drug dose 

-

-

-

Ischemic stroke

0

0

0

Hemorrhagic conversion

0

0

0

Major NMCR bleeding

 

0

0

Morality

0

0

0

Greater than 1 week from last study drug dose

-

-

-

Ischemic stroke

0

0

0

Hemorrhagic conversion

1

0

1

Major NMCR bleeding

0

0

0

Morality

3

1

2

 

Adverse Events

Common Adverse Events: N/A

Serious Adverse Events: N/A

Percentage that Discontinued due to Adverse Events: N/A

Study Author Conclusions

Time since the last dose may impact the outcomes in patients on NOACs who receive emergent thrombolysis

InpharmD Researcher Critique

The small sample size with no statistical analysis are limitations to this report. Patients included had complex clinical situations and the outcomes may have been impacted by their clinical status (vs. drug effect).

References:

Chen ST, Hellkamp AS, Becker RC, et al,. Outcome of patients receiving thrombolytic therapy while on rivaroxaban for nonvalvular atrial fibrillation (from rivaroxaban once daily oral direct factor xa inhibition compared with vitamin k antagonism for prevention of stroke and embolism trial in atrial fibrillation). Am J Cardiol. 2017;120(10):1837-1840.