Can venetoclax be crushed? If so, how long is the drug stable for?

Comment by InpharmD Researcher

The package insert for venetoclax states not to chew, crush, or break tablets prior to swallowing. However, crushing venetoclax has been attempted in limited studies, including a crossover study reporting similar bioavailability and plasma concentrations between crushed and intact venetoclax tablets. This was contradicted in a 2022 case report which described elevated plasma concentrations in a patient using crushed venetoclax tablets, which was thought to lead to neutropenia. Stability data for crushed tablets is limited to the previously mentioned crossover study, which saw no change in physicochemical properties in crushed or ground tablets after 72 hours when stored at 25°C/60% relative humidity; no data is available past 72 hours. Although there is an ongoing pharmacokinetic trial evaluating the administration of crushed venetoclax tablets in patients with an NG/G-tube, the study is estimated to be complete in 2026.

Background

A 2023 review article discussed the clinical pharmacokinetics and pharmacodynamics of venetoclax, a BCL-2 inhibitor approved for various hematological malignancies. Venetoclax is noted to have challenging physicochemical properties as it has low aqueous solubility. This has posed challenges for drug formulation and administration. The impact of crushing or grinding venetoclax tablets on its bioavailability was evaluated in a three-way, crossover study in healthy adults (see Table 1). Crushed and finely ground venetoclax tablets had similar bioavailability to intact venetoclax tablets, with only a slightly lower peak plasma concentration, which was not considered clinically significant. Therefore, the authors consider crushing or grinding venetoclax tablets to be a viable alternative method of administration for patients who have difficulty swallowing whole tablets. [1]

A 2022 case report describes a 74-year-old man with acute myeloid leukemia (AML) who experienced increased blood levels of venetoclax after ingesting crushed venetoclax tablets. Initially, the patient achieved remission with induction therapy consisting of idarubicin and cytarabine but later received venetoclax plus azacitidine due to intolerance to potent consolidation therapy. During the first cycle of home treatment, the patient reported difficulty swallowing and consequently crushed the venetoclax tablets for ingestion (approximately from days 19 to 32). However, starting with the second cycle, the patient was able to swallow the tablets whole. The median Cmin during the second cycle remained at 1.46 µg/mL (range 1.34 to 2.68), except on day 2 (day 34; 4.30 µg/mL). The patient developed severe, prolonged neutropenia during the second cycle that was persistently below 100/µL. The elevated plasma venetoclax concentration in this case may be attributed to the crushing of the tablets, as the Cmin of the uncrushed tablet was approximately twice that of the crushed tablet. Since venetoclax is a film-coated tablet and film-coated tablets typically have minimal pharmacokinetic effects when crushed, pharmaceutical companies do not currently have pharmacokinetic data for crushed tablets. Overall, the authors discourage the practice of crushing venetoclax tablets. [2]

Although a retrospective study from 2023 did not address the stability of crushed venetoclax tablets, it did indicate that the crushed form was used in pediatric patients. Venetoclax was administered orally as a tablet once daily with food if tolerated. However, for younger patients who could not swallow pills, the tablets were compounded in an oral hazardous compounding hood by crushing and dissolving them in sterile water to a final concentration of 5 mg/mL. Safety or stability concerns related to the administration of crushed tablets were not discussed. While this study did not assess the stability of Venetoclax being crushed, it highlights the approach taken to accommodate patient needs. [3]

Cincinnati Children’s is currently conducting a pharmacokinetic study of crushed venetoclax tablets dissolved in liquid solution in children and young adults with hematologic malignancies. Patients under 39 years old with any hematologic malignancy, weighing at least 5.5 kg, and having a central line for PK blood draws are eligible for the study. They must be receiving venetoclax, either alone or with other chemotherapeutic agents, as a solution made from crushed tablets administered orally or via NG/G-tube. The study is estimated to be completed in 2026 with 30 enrolled patients. [4], [5]

References:

[1] Salem AH, Menon RM. Clinical pharmacokinetics and pharmacodynamics of venetoclax, a selective B-cell lymphoma-2 inhibitor. Clin Transl Sci. 2024;17(5):e13807. doi:10.1111/cts.13807
[2] Anzai M, Yasu T, Gando Y, Shirota M, Kobayashi M. Increased blood levels of venetoclax due to intake of crushed venetoclax tablets. Ann Hematol. 2022;101(9):2097-2098. doi:10.1007/s00277-022-04871-2
[3] Marinoff AE, Aaronson K, Agrawal AK, et al. Venetoclax in combination with chemotherapy as treatment for pediatric advanced hematologic malignancies. Pediatric Blood & Cancer. 2023;70(6):e30335. doi:10.1002/pbc.30335
[4] Cincinnati Children’s. Pharmacokinetic Study of Venetoclax Tablets Crushed and Dissolved Into a Solution. Accessed August 30, 2024. https://www.cincinnatichildrens.org/service/c/clinical-trials/studies/venetoclax-PK-112223
[5] ClinicalTrials.gov. Pharmacokinetic Study of Venetoclax Tablets Crushed and Dissolved Into a Solution. Updated July 10, 2024. Accessed August 30, 2024. https://clinicaltrials.gov/study/NCT06131801

Relevant Prescribing Information

Administration
Instruct patients of the following:
Take VENCLEXTA with a meal and water.
Take VENCLEXTA at approximately the same time each day.
Swallow VENCLEXTA tablets whole. Do not chew, crush, or break tablets prior to swallowing. [6]

References:

[6] Venetoclax tablet. Prescribing information. AbbVie Inc.; 2024.

Literature Review

A search of the published medical literature revealed 1 study investigating the researchable question:

Can venetoclax be crushed? If so, how long is the drug stable for?

Level of evidence

C - Multiple studies with limitations or conflicting results  Read more→



Please see Table 1 for your response.


 

Venetoclax Clinical Pharmacokinetics After Administration of Crushed, Ground, or Whole Tablets

Design

Open-label, randomized, 3-way crossover study 

N= 15

Objective

To evaluate the safety, stability, and oral bioavailability of venetoclax tablets in crushed and finely ground forms relative to intact tablets

Study Groups

Intact (N= 15)

Crushed (N= 15)

Finely ground (N= 15)

Inclusion Criteria

Healthy adult females aged 18 to 65 y/o; postmenopausal or permanently surgically sterile; or premenopausal practicing at least 2 methods of birth control

Exclusion Criteria

Males

Methods

Enrolled females were randomly assigned 1:1:1 into three groups (crushed, intact or ground) to receive a single dose of one of the three regimens in each period. Venetoclax 100 mg tablet was administered orally with water (240 mL) following a high-fat breakfast. Blood samples were collected up to 72 hours post-dosing. Participants were randomized to the other groups after a 5-day washout between treatments.

The crushed tablets were prepared using a manual pill crusher, and the finely ground tablets were prepared with an automatic pill crusher. Both forms were transferred to an amber vial and stored at room temperature before administration. The experimental groups were administered the fine or coarse powder by mouth directly from the amber vials followed by a glass of water within 30-minutes of breakfast.

Duration

15 days total, with a 5-day washout period between each dosing period

Outcome Measures

Primary: Area under the curve from time zero to the time of the last measurable concentration (AUCt), AUC extrapolated to infinite time (AUCinf), maximum plasma concentration (Cmax), time to Cmax (Tmax), terminal half-life (t1/2)

Secondary: Safety (via vital signs and electrocardiogram); stability

Baseline Characteristics   Healthy females (N= 15)

Median age, years (range)

46 (25 to 65)
Median BMI, kg/m2

27.7

Race

Black/African American

White

Multiracial

 

73.3%

20%

6.7%

Results Pharmacokinetic Parameters Intact Tablet Crushed Tablet Ground Tablet

Cmax, μg/mL

0.873 0.776 0.646

Tmax, h (range)

6 (4 to 10) 6 (4 to 6) 6 (4 to 10)

t1/2, h

14.1 ± 2.05 13.3 ± 1.85 11.9 ± 1.53

AUCt, μg·h/mL

10.2 10.1 10.4

AUCinf, μg·h/mL

10.4 10.3 10.6

There was no change in the physical appearance and the evaluated physicochemical properties of crushed and ground venetoclax tablets after 72 hours of storage at 25°C/60% relative humidity. The water content of the ground tablets did not change significantly from time 0 to 72 hours, but the crushed tablet samples showed a small moisture uptake of 0.5 to 1.0% w/w at 72 hours when stored under the same conditions.

Adverse Events

1 participant reported nausea (Grade 1) following administration of intact venetoclax tablet. No other adverse events or clinically meaningful abnormalities were observed.

Study Author Conclusions

Crushing or grinding venetoclax tablets before administration could be considered as a viable alternative method of administration for patients who have difficulty swallowing whole venetoclax tablets or patients requiring nasogastric tube feeding.

Critique

This study featured a well-designed crossover approach, comprehensive pharmacokinetic analysis, and thorough stability assessment. However, the small sample size limited its power to establish bioequivalence, and the exclusion of male participants due to potential toxicity may impact the generalizability of the findings.

 

References:

Alaei S, Wang Y, Liu Y, et al. Venetoclax Clinical Pharmacokinetics After Administration of Crushed, Ground or Whole Tablets. Clin Ther. Published online May 22, 2024. doi:10.1016/j.clinthera.2024.03.012