Can acetylcysteine be used in an esophagogastroduodenoscopy (EGD) procedure?

Comment by InpharmD Researcher

Available data evaluating the use of N-acetylcysteine (NAC) in the setting of esophagogastroduodenoscopy (EGD) procedure suggest NAC in combination with simethicone to be superior to simethicone alone or placebo/control for improving mucosal visibility and diagnostic yield. Notably, NAC alone does not appear to have been studied for EGD; it was always used with simethicone.

Background

N-acetylcysteine (NAC) has been used as premedication for gastric visualization procedures due to its mucolytic activity (to reduce gastric mucin and lower its viscosity). A 2025 meta-analysis systematically reviewed 20 randomized controlled trials (RCTs; 10 RCTs specifically involved a combination regimen) investigating the efficacy of simethicone, with or without NAC, as premedication for esophagogastroduodenoscopy (EGD). The analysis included 8,893 participants from diverse geographic regions, with interventions involving simethicone dosages ranging from 20 mg to 400 mg and NAC dosages from 300 mg to 1000 mg. All premedications were administered as a single dose 15 to 30 minutes prior to EGD. The primary outcome assessed was mucosal visibility, while secondary outcomes included procedure time, required cleansing volume, lesion detection rate, and safety profile. According to the findings, simethicone with or without NAC significantly improved mucosal visibility compared to controls (standardized mean differences [SMD] -1.27; 95% confidence interval [CI] -1.74 to -0.81; p<0.001), with the combination of simethicone and NAC yielding superior results to simethicone alone (SMD -0.68; 95% CI -1.08 to -0.28; p= 0.001). The intervention also reduced procedure time compared to control (mean differences [MD] -1.40, 95% CI -2.67 to -0.12, p= 0.03) and decreased the required cleansing volume (MD -29.12; 95% CI -50.60 to -7.64; p= 0.008). However, the lesion detection rate remained similar across groups (risk ratio [RR] 1.21; 95% CI 1.07-1.36; p= 0.002). The analysis identified a low overall risk of bias, but potential publication bias was noted. Safety evaluations indicated no increase in adverse events, with fewer reported side effects in the simethicone groups compared to control (RR 0.60, 95% CI 0.38-0.94, p= 0.03). These findings support the use of simethicone, particularly in combination with NAC, as an effective and well-tolerated premedication to enhance mucosal visibility and optimize procedural efficiency in EGD. [1]

Another meta-analysis and systematic review, this one published in 2019, evaluated the efficacy and safety of simethicone with or without NAC as premedication before gastroscopy using data from 10 RCTs (N= 5,750). Patients were administered either simethicone alone, simethicone plus NAC, or water as a control, with oral intake occurring 5 to 30 minutes before the procedure. Findings demonstrated that simethicone plus NAC significantly improved the rate of positive findings compared to water (RR 1.31; 95% CI 1.12 to 1.53; p= 0.0006), whereas no significant difference was observed between simethicone alone and water (RR 1.13; 95% CI 0.82 to 1.55, p = 0.46). Mucosal visibility scores were superior in both the simethicone alone and simethicone plus NAC groups compared to water, with the latter combination providing greater benefit (MD -0.14; 95% CI -0.25 to -0.03; p= 0.01). Additionally, procedure time was significantly shorter in the simethicone group than in the water group (MD -1.23 min; 95% CI -1.51 to -0.96; p<0.00001). No significant difference in adverse events was observed between simethicone and water (RR 0.45; 95% CI 0.2 to 1.0; p= 0.05). The findings suggest that simethicone, particularly in combination with NAC, enhances mucosal visibility and diagnostic yield while maintaining a favorable safety profile. [2]

References:

[1] Tanadi C, Pajala FB, Supranoto YTN, et al. Simethicone with or without N-acetylcysteine as premedication in esophagogastroduodenoscopy: a systematic review and meta-analysis. Ann Gastroenterol. 2025;38(1):28-40. doi:10.20524/aog.2024.0930
[2] Li Y, Du F, Fu D. The effect of using simethicone with or without N-acetylcysteine before gastroscopy: A meta-analysis and systemic review. Saudi J Gastroenterol. 2019;25(4):218-228. doi:10.4103/sjg.SJG_538_18

Literature Review

A search of the published medical literature revealed 3 studies investigating the researchable question:

Can acetylcysteine be used in an esophagogastroduodenoscopy (EGD) procedure?

Level of evidence

B - One high-quality study or multiple studies with limitations  Read more→



Please see Tables 1-3 for your response.


 

Simethicone and N-acetyl Cysteine Combination as Premedication Before Esophagogastroduodenoscopy: Double-blind Randomized Controlled Trial
Design

Prospective, double-blind, randomized placebo-controlled trial in a 2 × 2 factorial design

N=768

Objective

To detect whether the combination of simethicone with N-acetyl cysteine (NAC) as premedication before EGD improves mucosal visualization

Study Groups

Group A: simethicone + NAC (n= 192)

Group B: simethicone alone (n= 192)

Group C: NAC alone (n= 192)

Group D: placebo (n= 192)

Inclusion Criteria All adults > 18 years of age undergoing diagnostic EGD
Exclusion Criteria

Patients with partial or complete gastric outlet obstruction, minimum fasting <6 hours, history of active/recent gastrointestinal bleed including melena, pregnancy, history of post-gastric surgery, phenylketonuria, or suspected hypersensitivity to the study drugs

Methods

Participants drank 90 mL of solution (15 mL emulsion + 75 mL water) 20 minutes before EGD. Group A received simethicone 150 mg + NAC 600 mg, Group B received simethicone 150 mg, Group C received NAC 600 mg, and Group D received placebo. EGD was performed and images were obtained from the esophagus, stomach, and duodenum. Mucosal visibility was scored from 1 to 4, and the total mucosal visibility score (TMVS) was calculated

Duration

September 2017 to March 2018

Outcome Measures

Primary: Total mucosal visibility score (TMVS)

Secondary: Total duration of the procedure, amount of water used to flush mucous/bubbles, need for suction, lesions detected, patient comfort

Baseline Characteristics   Group A (n= 192) Group B (n= 192) Group C (n= 192)

Group D (n= 192)

Age, years

45.46 ± 14.56 44.45 ± 14.59 43.42 ± 13.55 43.40 ± 13.09

Male

106 (55.2%) 106 (55.2%) 125 (65.1%) 101 (52.6%)
Results

 

Group A Group B Group C Group D p-value

Total Mucosal Visibility score

8.31 ± 1.73 9.93 ± 2.19 11.46 ± 2.4 12.06 ± 2.3 <0.05

No flushing/suction required

81% 68% 50%* 27% <0.05

Good-to-tolerable discomfort

98% 98% 96% 86% --
Adverse Events

No adverse effects were noted on using the study drug for study participants

Study Author Conclusions

Using simethicone and NAC combined for premedication may improve the quality of EGD by increasing gastric mucosal visibility.

Critique

The study was a large, randomized placebo-controlled trial, which is a strength. However, TMVS is subjective and may vary with individual endoscopists. The study did not address whether improved visualization leads to superior detection of early mucosal lesions, which is a limitation.

 

References:

Krishnamurthy V, Joseph A, Venkataraman S, Kurian G. Simethicone and N-acetyl cysteine combination as premedication before esophagogastroduodenoscopy: Double-blind randomized controlled trial. Endosc Int Open. 2022;10(5):E585-E592. Published 2022 May 13. doi:10.1055/a-1782-2003

 

Premedication with Simethicone and N-acetylcysteine in Improving Visibility During Upper Endoscopy: a Double-blind Randomized Trial
Design

Randomized, single-center, double-blind, placebo-controlled trial

N= 297

Objective

To assess the efficacy of premedication with simethicone alone or in combination with N-acetylcysteine in improving mucosal visibility during upper endoscopy

Study Groups

Group A (placebo; n= 98)

Group B (water + simethicone; n= 101)

Group C (water + simethicone + N-acetylcysteine; n= 98)

Inclusion Criteria

Patients scheduled for upper GI endoscopy

Exclusion Criteria

Patients undergoing the procedure with sedation, previous total gastrectomy, known neoplasia or stenosis, allergies to simethicone or N-acetylcysteine, undergoing therapeutic or urgent procedure

Methods

Patients were randomized to receive premedication 15–30 minutes before endoscopy: Group A received 100mL of water (placebo), Group B received 100mL of water plus 100mg simethicone, and Group C received 100mL of water plus 100mg simethicone plus 600mg N-acetylcysteine. Endoscopic visibility was scored as excellent, adequate, or inadequate.

Duration

April to December 2015

Outcome Measures

Primary: Quality rating of mucosal visualization in the esophagus, stomach, and duodenum

Secondary: Rating using a three-point scale for visualization quality in patients with previous partial gastrectomy, identification of mucosal lesions by organ

Baseline Characteristics   Group A (n= 98) Group B (n= 101)

Group C (n= 98)

Age, years 64 ± 12 62 ± 16

62 ± 14

Male 48 (49.0%) 47 (46.5%) 54 (55.1%)
Female 50 (51.0%) 54 (53.5%) 44 (44.9%)
Diabetes mellitus 18 (18.4%) 14 (13.9%) 17 (17.3%)
Previous gastric surgery 21 (21.4%) 20 (19.8%) 19 (19.4%)
Results   Group A (n= 98) Group B (n= 101)

Group C (n= 98)

p-value
Esophagus - Excellent 70 (71.4%) 92 (91.1%) 85 (86.7%) <0.001
Stomach - Excellent 38 (38.8%) 77 (76.2%) 73 (74.5%) <0.001
Duodenum - Excellent 72 (73.5%) 86 (85.1%) 80 (81.6%) 0.042
Adverse Events

Only one adverse event occurred (0.3%) in a patient from group A, who vomited during endoscopy. No further complications occurred

Study Author Conclusions

Premedication with simethicone resulted in better mucosal visibility and might improve diagnostic yield, suggesting it should be considered for standard practice. The addition of N-acetylcysteine requires further investigation.

Critique

The study demonstrated the benefit of simethicone premedication in improving mucosal visibility during endoscopy. However, it was underpowered to detect differences between active treatments and was conducted at a single center, which may limit generalizability. The lack of a standard scale for mucosal visualization and the absence of investigation into differences in procedure time and patient tolerance are limitations.

 

References:

Elvas L, Areia M, Brito D, Alves S, Saraiva S, Cadime AT. Premedication with simethicone and N-acetylcysteine in improving visibility during upper endoscopy: a double-blind randomized trial. Endoscopy. 2017;49(2):139-145. doi:10.1055/s-0042-119034

 

Use of N-acetylcysteine Plus Simethicone to Improve Mucosal Visibility During Upper GI Endoscopy: a Double-blind, Randomized Controlled Trial
Design

Randomized, double-blinded, placebo-controlled trial

N= 230

Objective

To determine whether premedication with simethicone or simethicone plus N-acetylcysteine is effective in improving visibility during upper GI endoscopy (UGE)

Study Groups

No intervention (n= 46)

Water (n= 46)

Simethicone 200 mg (n= 46)

Simethicone + N-acetylcysteine 500 mg (n= 46)

Simethicone + N-acetylcysteine 1000 mg (n= 46)

Inclusion Criteria

Adults undergoing ambulatory diagnostic UGE

Exclusion Criteria

History of upper GI tract surgery, gastric cancer, deep sedation with propofol, need for therapeutic endoscopy and emergency procedures, recent upper GI bleeding, caustic ingestion, pregnancy, diabetes mellitus, asthma, and allergic reactions to the medications used in the study

Methods

Patients were randomized into five groups: no intervention, water, simethicone 200 mg, simethicone + NAC 500 mg, and simethicone + NAC 1000 mg. Solutions were ingested 20 minutes before UGE. Gastric visibility was evaluated in four segments using a scale. Adequate visibility was defined as a score of less than 7 points. Water volume used to improve visibility.

Duration

July 2012 to August 2013

Outcome Measures

Primary: Adequate visibility (AV) defined as gastric total visibility scale (TVS) <7 points

Secondary: Amount of water used to improve visibility, time to complete UGE, adverse reactions

Baseline Characteristics   No intervention (n= 46) Water (n= 46) Simethicone (n= 46) S+NAC500 (n= 46)

S+NAC1000 (n= 46)

Female

44% 41% 46% 65% 67%

Age, years (IQR)

55 (41-70) 47.5 (33-68) 48.5 (33-77) 48 (41-70) 46 (31-70)
Results  

No intervention (n= 46)

Water (n= 46) Simethicone (n= 46) S+NAC500 (n= 46) S+NAC1000 (n= 46) p-value
Adequate visibility 44% 41% 46% 65% 67% <0.05

Median TVS (IQR)

7 (5-12) 7 (6-14) 7 (5-10) 6 (5-10) 5 (4-11) <0.05

Compared with water, the simethicone + NAC 500 mg (p= 0.03) and NAC 1000 mg (p= 0.008) had significant improvements via gastric total visibility scale (TVS); simethicone alone was not significantly different than water (p= 0.14) and no intervention.

NAC 1000 mg added to simethicone had significantly better visibility compared to simethicone alone (p= 0.046), but the NAC 500 mg group was not significantly different (p= 0.081).

The simethicone + NAC 1000 mg group required was also significantly lower in the simethicone + NAC 1000 mg group (p= 0.035), but the volume of water used per group were not reported.

Adverse Events

No adverse reactions from the study drugs were observed

Study Author Conclusions

Premedication with S+NAC500 and S+NAC1000 improves visibility during UGE. The use of simethicone alone did not show improvements in gastric visibility.

Critique

The study was well-designed with a clear objective and robust methodology. However, the lack of a premedication comparison for each patient limits the assessment of individual preparation impact. The study's findings on lesion detection were incidental and not the primary focus, suggesting the need for further investigation into clinical outcomes.

 

References:

Monrroy H, Vargas JI, Glasinovic E, et al. Use of N-acetylcysteine plus simethicone to improve mucosal visibility during upper GI endoscopy: a double-blind, randomized controlled trial. Gastrointest Endosc. 2018;87(4):986-993. doi:10.1016/j.gie.2017.10.005