Summarize the data on formaldehyde use for hemorrhagic cystitis

Comment by InpharmD Researcher

Available data on the use of formaldehyde (formalin solution) for hemorrhagic cystitis (HC) is primarily limited to small retrospective studies and older case reports, and formall guidance on its use is scarce. One guideline by the Canadian Urological Association, as well as several clinical practice reviews, consistently describe intravesical formalin as a last-line intervention for refractory HC, emphasizing that it is typically considered only after other medical and procedural therapies have failed and prior to surgical management. Reported regimens most commonly use 4% formalin, which often achieves hemostasis after a single instillation (usually with a dwell time of ≤15 minutes), although lower concentrations (1-2%) may also be effective but often require multiple instillations. Data have described success rates of 70-90% with formalin treatment, however it carries significant risk, especially at higher concentrations (e.g., 10%), such as ureteral strictures, bladder contracture, vesicovaginal fistula, and vesicorectal fistula, all potentially leading to long-term bladder dysfunction, as well as renal failure, and rare formalin‑related deaths. Overall, despite formalin’s hemostatic efficacy, there is limited quality of evidence and potential for serious complications underscoring the need for careful patient selection and reserving therapy for cases in which other treatment options have failed.

Background

A 2019 Canadian Urological Association best practice report on the management of hemorrhagic cystitis in the pediatric population states that clinicians can consider sclerotherapy with formalin in severe, refractory cases, but as a last resort prior to irreversible surgical options (Level 3 evidence, Grade D recommendation). Given that instillation is painful, general anesthesia is required and it is recommended that all patients undergo evaluation for vesicoureteral reflux (VUR) beforehand to prevent upper-tract injury. If VUR is present, ureteral orifices may be temporarily occluded during treatment with Fogarty catheters. Reports using 4% formalin have been effective with some children improving after a single treatment while lower concentrations (1-2%) have been effective but required multiple instillations. The guideline emphasizes that due to potential long-term bladder compromise (e.g., bladder scarring), formalin should be typically avoided or use as a last resort, especially in the pediatric population. [1]

Recent reviews consistently describe formalin as a last‑line intervention for severe, refractory hemorrhagic cystitis due to its significant toxicity profile and lack of guideline endorsement. Its hemostatic efficacy and clinical outcomes vary widely, with reported response rates ranging from 75-89%, however, often followed by recurrence or progression to bladder dysfunction. Lower concentrations (1-2%) may achieve control but frequently require repeat instillations, whereas higher concentrations (≥4%) correlate with greater systemic absorption, ureteral injury, severe fibrosis, and increased risk of major complications, including ureteral strictures, bladder contracture, vesicovaginal fistula, vesicorectal fistula, and even formalin‑related deaths. Several studies report that up to 31-40% of patients ultimately require urinary diversion or cystectomy because of formalin‑related damage. Formalin exposure also raises theoretical carcinogenic concerns due to formaldehyde classification, though long‑term cancer risk data remain insufficient. Overall, while formalin can rapidly control life‑threatening hematuria, its dose‑dependent toxicity and risk of irreversible injury limit its use to carefully selected cases when all safer options have failed. [2], [3], [4]

Additional reviews highlight that given radiotherapy commonly involves cancers in the lower abdomen and pelvis, the bladder and urinary tract are frequently exposed to radiation increasing the risk of developing radiation-induced hemorrhagic cystitis. Bladder instillation with formalin is again discussed as a treatment option for clinically significant gross hematuria or hemorrhagic cystitis only when other supportive measures and treatments have failed and surgery is being considered. One review notes that while formalin concentrations of 1-10% have been used, maximum dilution is preferred due to increased toxicity with higher concentration and that the instillation should not exceed 15 minutes. Treatment success with formalin has ranged from 70-90%; however, its use is associated with major complications such as ureter obstruction, renal failure, and formalin reflux into the upper urinary tract leading to bilateral pyonephrosis with lethal sepsis. [5], [6]

A 1990 retrospective review evaluated the treatment and clinical outcomes of a cohort of 25 patients (mean age 65 years) with massive hemorrhagic cystitis who received intravesical instillation of formalin from 1980 to 1988. The hemorrhagic episodes primarily stemmed from pelvic radiotherapy in 15 cases, cyclophosphamide therapy in one case, and infiltrating bladder cancer in the remaining nine cases. Of these patients, 19 were treated with a 4% formalin solution, while the remaining six received a 10% solution. The instillation was performed, under general or intradural anesthesia, with a maximum contact time of 15 minutes in 80% of patients. Notably, 10 patients underwent the procedure with prior supravesical urinary diversion, such as ileal conduits or percutaneous nephrostomies, which accounted for approximately 40% of the treatments conducted in 'excluded' bladders (without passage of urine). Immediately after the instillation with formalin, bladder lavage was performed using 10% alcohol and a continuous bladder irrigation with normal saline for 24-48 hours. The review documented good outcomes in 88% of cases, with effective hemostasis achieved for an average duration of four months. Complications were more frequent with the use of the 10% formalin concentration. Specifically, one case each of vesicorectal fistula, uretero-hydronephrosis, and vesical extravasation of formalin were noted when higher concentration and volume were used. In contrast, the use of 4% formalin was associated with a complication of upper urinary tract dilatation in only one instance. These findings underscore that lower concentrations and smaller volumes of formalin result in fewer complications, advocating the use of 4% formalin for managing massive hemorrhagic cystitis when conservative measures fail to control bleeding. [7]

References: [1] Hannick JH, Koyle MA. Canadian Urological Association Best Practice Report: Pediatric hemorrhagic cystitis. Can Urol Assoc J. 2019;13(11):E325-E334. doi:10.5489/cuaj.5993
[2] Mihailidis T, Davenport K. The Practical Management of Intractable Haematuria Within the National Health Service of the United Kingdom: A Literature Review. Cureus. 2025;17(11):e96083. Published 2025 Nov 4. doi:10.7759/cureus.96083
[3] Jefferson FA, Linder BJ. Hemorrhagic Cystitis: Making Rapid and Shrewd Clinical and Surgical Decisions for Improving Patient Outcomes. Res Rep Urol. 2023;15:291-303. Published 2023 Jun 29. doi:10.2147/RRU.S320684
[4] Petca RC, Popescu RI, Toma C, et al. Chemical hemorrhagic cystitis: Diagnostic and therapeutic pitfalls (Review). Exp Ther Med. 2021;21(6):624. doi:10.3892/etm.2021.10056
[5] Chorbińska J, Krajewski W, Zdrojowy R. Urological complications after radiation therapy-nothing ventured, nothing gained: a Narrative Review. Transl Cancer Res. 2021;10(2):1096-1118. doi:10.21037/tcr-20-2589
[6] Leddy LS. Management of Lower Urinary Tract Symptoms After Pelvic Radiation in Females. Curr Urol Rep. 2018;19(12):106. Published 2018 Oct 31. doi:10.1007/s11934-018-0848-2
[7] Vicente J, Rios G, Caffaratti J. Intravesical formalin for the treatment of massive hemorrhagic cystitis: retrospective review of 25 cases. Eur Urol. 1990;18(3):204-206. doi:10.1159/000463910
Literature Review

A search of the published medical literature revealed 2 studies investigating the researchable question:

Summarize the data on formaldehyde use for hemorrhagic cystitis

Level of evidence

C - Multiple studies with limitations or conflicting results  Read more→



Please see Tables 1-2 for your response.


Intravesical Formalin for Hemorrhagic Cystitis: A Contemporary Cohort
Design

Retrospective cohort study

N= 8

Objective To evaluate outcomes of a contemporary cohort of patients treated with intravesical formalin for hemorrhagic cystitis
Study Groups All patients (N= 8)
Inclusion Criteria Patients ≥18 years hospitalized for hemorrhagic cystitis between 2004 and 2014 who failed initial measures including indwelling catheter, continuous normal saline bladder irrigation, and intravesical therapy with agents such as alum, silver nitrate, and aminocaproic acid
Exclusion Criteria Not specified.
Methods Intravesical formalin instillation was performed under general anesthesia. Beforehand, rigid cystoscopy was used for clot evacuation and fulguration of any discrete bleeding vessels. Patients were also evaluated for bladder perforation and evidence of vesicoureteral reflux (VUR). External genitalia was protected from formalin exposure using plastic wrap after applying copious amounts of petroleum jelly and covered with sterile surgical towels to any remaining exposed areas. Formalin solution (1-4%) was instilled into the bladder under gravity with a dwell time of 10-15 minutes. After draining the formalin from the bladder, the bladder was irrigated with sterile water and then continuous bladder irrigation with normal saline was initiated.
Duration Retrospective chart review period: 2000 to 2014
Outcome Measures

Primary: Resolution of hematuria during hospitalization without additional invasive therapies

Secondary: Time to resolution, number of instillations, dwell time, incidence of reflux, 30-day treatment complications, additional procedures needed, 1-year and 3-year overall survival rates after formalin instillation

Baseline Characteristics   Intravesical formalin instillation (N= 8)
Age, median (IQR), years 83 (79.5–83.5)
Female 2 (25%)

Hemorrhagic cystitis etiology

XRT for PCa

XRT for GYN Ca

 

6 (75%)

2 (25%)

BMI, median (IQR) 30.2 (23.5–31.8)
Diabetes mellitus 1 (13%)
Hypertension 5 (63%)
Coronary artery disease 2 (25%)
Current or previous smoker 2 (25%)

Prior hemorrhagic cystitis treatment

Continuous bladder irrigation

Cystoscopy + clot evacuation

Intravesical alum

Silver nitrate

Aminocaproic acid

Hyperbaric oxygen therapy

 

8 (100%)

8 (100%)

6 (75%)

4 (50%)

2 (25%)

2 (25%)

Abbreviations: BMI= body mass index. IQR= interquartile range. GYN Ca= gynecological cancer. PCa= prostate cancer. XRT= external beam radiation.
Results   Intravesical formalin instillation (N= 8)

Total formalin instillations

1

2

3

 

5 (63%)

2 (25%)

1 (13%)

Dwell time, median (range), mins 10 (10–15)
Reflux 4 (50%)

30-day complications

Clavien I

Clavien II

Clavien III

Clavien IV

 

0 (0%)

5 (63%)

0 (0%)

1 (13%)

Resolution of hematuria 6 (75%)
Time to resolution, median (range), days 4 (1–17)

Additional procedures needed

Bilateral nephrostomy tube placement

Cystectomy with urinary diversion

4 (50%)

1 (13%)

3 (38%)

1-year overall survival rate

75%

3-year overall survival rate

50%

Adverse Events

Severe complications occurred in one patient, including postoperative acute kidney injury and respiratory distress requiring a stay in the intensive care unit (classified as Clavien IVa complications).

Five patients required blood transfusion within 30 days of formalin instillation (classified as Clavien II complication).

One patient who was treated with formalin dose escalation ultimately developed a severely contracted bladder with refractory urinary urgency, urge incontinence, and recurrent bladder neck contracture and underwent delayed planned cystectomy for refractory symptoms approximately eight months after formalin instillation.

Four patients died unrelated to their hemorrhagic cystitis.

Study Author Conclusions Formalin remains an important tool for treating refractory hemorrhagic cystitis, with roughly 75.0% of patients requiring no additional therapy prior to hospital discharge. Notably, there is a risk of bladder dysfunction following formalin.
Critique The study is limited by its small cohort size and retrospective design, which may introduce bias. The lack of a standardized follow-up protocol and the involvement of multiple surgeons over a long time span may affect the consistency of treatment outcomes. Despite these limitations, the study provides valuable insights into the use of intravesical formalin for hemorrhagic cystitis in a contemporary cohort.

 

References:
[1] Ziegelmann MJ, Boorjian SA, Joyce DD, Montgomery BD, Linder BJ. Intravesical formalin for hemorrhagic cystitis: A contemporary cohort. Can Urol Assoc J. 2017;11(3-4):E79-E82. doi:10.5489/cuaj.4047

 

Formalin Treatment of Refractory Hemorrhagic Cystitis

Design

 Case Report

Case presentation

 A 14-year-old boy underwent high-dose chemotherapy and resection for chest-wall rhabdomyosarcoma. Despite prophylactic measures including MESNA and hydration therapy, postoperative high-dose chemotherapy, including cyclophosphamide with stem-cell rescue, caused the patient to develop significant gross hematuria, complicating into painful clot retention and renal function deterioration. Conservative interventions such as bladder irrigation and cystoscopic clot evacuations were unsuccessful. Cystotomy revealed grossly hemorrhagic and edematous bladder mucosa. Additionally, ureteric stents were installed and the bladder was packed with gauze swabs for several days, however upon their removal, the bleeding continued to the degree of requiring multiple transfusions.

After considering cystectomy, an unconventional approach was adopted using intravesical formalin treatment. Swabs soaked in 4% formalin were applied to the bladder mucosa for several minutes, resulting in rapid cessation of bleeding. Following closure of the bladder and removal of ureteric stents, the patient experienced no voiding abnormalities or renal dysfunction, and over a 36-month follow-up, there was no recurrence of sarcoma.

Author discussion within the same report highlighted that historically the control of intractable hemorrhagic cystitis using formalin is initially effective in advanced bladder carcinoma cases. Nevertheless, they cautioned that the method is associated with potential morbidity, including ureteric stenosis and renal complications, stemming from formaldehyde absorption.

Study Author Conclusions

Intravesical formalin may obviate the need for radical surgery in the face of failure of other conservative measures. Open instillation is favored in order to reduce the risk of complications.

 

References:
[1] [1] Joseph CM, Bowley DM, Pitcher GJ. Formalin treatment of refractory hemorrhagic cystitis. J Pediatr Urol. 2005;1(5):365-367. doi:10.1016/j.jpurol.2005.02.005