How is IV ketamine administered for treatment-resistant depression?

Comment by InpharmD Researcher

Studies of ketamine infusions for treatment-resistant depression typically administered 0.5 mg/kg over 40 minutes; some studies have also evaluated 0.2 mg/kg and 1 mg/kg. Higher doses (0.5-1 mg/kg) appear to be superior to lower doses in efficacy, and single-dose studies indicate no superiority of 1 mg/kg over 0.5 mg/kg. A recent proof-of-concept study found a 96-hour infusion of ketamine (target rate of 0.6 mg/kg/h) induced remission that was sustained for up to 8 weeks in adults with treatment-resistant depression. While the ideal frequency of ketamine infusion is not known, patients who respond to initial treatment may benefit from multiple infusions up to 0.5 mg/kg given 2-3 times/week over 2 weeks.

Background

A 2019 review of reviews, meta-analyses, and randomized controlled trials (not included in the other reviewed literature) found that low-dose intravenous ketamine provides a rapid antidepressant effect at 24 hours. Validated depression rating scales showed that administration of ketamine changed scores in as little as 40 mins with effects lasting at least 1-2 weeks. Still, there is limited and inconsistent data for ketamine having efficacy in depressive patients after 2 weeks. Remission symptoms have been observed at 80 mins, 1 day, and up to 2 weeks. Two studies showed a reduction of suicidal thoughts after 1-2 days after a single dose of ketamine, but this effect was not seen after 3 weeks in a third study. Studied doses of ketamine included 0.1 mg/kg, 0.2 mg/kg, 0.5 mg/kg, 1 mg/kg, and 2 mg/kg. Most infusion times reported were over 40 minutes, with one study delivering six 0.5 mg/kg infusions over 45 mins thrice weekly for three weeks (no efficacy was observed in this study). [1], [2], [3]

Ketamine as an antidepressant was shown to be well tolerated and adverse events usually occurred with very high doses administered over an extended period of time. Some of the most common adverse events were dizziness, dissociation, nausea, headaches, vertigo, and dysgeusia which usually resolve spontaneously. No long-term psychotomimetic side effects have been reported in any o the studies, but one isolated case of suicide attempt was reported in one study. The dose, mode of administration, and long-term effects of ketamine need to be further explored to optimize its antidepressant effects. [1], [2], [3]

A 2020 meta-analysis evaluated the effects of intravenous (IV) ketamine infusion in patients with treatment-resistant depression (TRD) using data from 19 studies (7 randomized controlled trials and 12 open-label trials; N= 818). All included studies utilized a single dose of IV ketamine infusion of 0.5 mg/kg, with outcomes measured up to 7 days post-infusion. The overall effects of ketamine were noted as early as 4 hours, peaked at 24 hours, but gradually diminished from days 2 to 7. Subgroup analysis of depression scores in patients receiving ketamine compared to placebo demonstrated a standard mean difference (SMD) of 0.77 (95% CI 0.46 to 1.08) at 24 hours and 0.49 (95% 0.20 to 0.78) at 7 days. Overall, IV ketamine was associated with a significantly improved SMD in depression scores (0.68; 95% 0.46 to 0.90). Similarly, better clinical response and clinical remission were also observed in ketamine recipients. Though a quantitative analysis was not performed for multiple versus single ketamine infusions, results demonstrated that multiple infusions (0.5 mg/kg, 3 times/week over 2 weeks) led to enhanced effectiveness and delayed clinical relapse. This was also observed when ketamine was used in a maintenance schedule with patients who responded to the initial treatment. However, long-term data are still required to investigate the safety and efficacy of ketamine in patients presenting with resistant symptoms. [5]

An international group of mood disorder experts provided a synthesis of the literature regarding the safety, efficacy, and tolerability of ketamine and esketamine in adults with treatment-resistant depression. It was recommended to start the administration of IV ketamine at a dose of 0.5 mg/kg with an infusion time of 40 minutes. While the efficacy at lower doses (0.1-0.2 mg/kg) may be inferior, doses may be lowered in cases of intolerability. The upper dosing limit for ketamine infusions in this patient population is not established, but studies show no evidence of superiority of 1 mg/kg over 0.5 mg/kg. The panel recommended the utilization of ideal body weight in patients who are overweight or obese, as this patient population may show a higher response rate to ketamine. A follow-up assessment should be conducted after completing four to six IV infusions to determine the intervention's overall efficacy. If the individual presented with a minimal response (i.e., ≤20% improvement from baseline in total depression symptom severity) after four to six infusions, that individual would be considered non-responsive and further infusions would not be warranted. There is insufficient data regarding the dosing and frequency of the administration of IV ketamine as a maintenance treatment, but studies have also shown no difference in efficacy between twice-weekly and thrice-weekly 0.5 mg/kg infusions over 40 minutes. It was suggested that practitioners perform periodic evaluations of the need for ongoing treatment on a monthly to bi-monthly basis. [4]

A proof-of-concept study published in 2021 evaluated prolonged ketamine infusions for treatment-resistant depression in 27 adults. Eligible participants were admitted for 5 days to receive a continuous 96-hour infusion of ketamine, started at 0.15 mg/kg/hour and titrated twice daily (as tolerated) to a target rate of 0.6 mg/kg/hour. This titration was based on an expected plasma concentration of 400 ng/mL, corresponding to an estimated 50% blockade of NMDA receptors. All patients were also started on PO clonidine 0.1 mg (titrated to 0.3 mg) approximately a week before the infusion; clonidine was stopped at the completion of the infusion. Side effects were generally mild and improved over the course of the infusion. Resulting Montgomery-Asberg Depression Rating Scale (MADRS) scores significantly fell after the ketamine infusion before slowly rising over the next 8 weeks. One day after the infusion, 59% of patients were in remission (MADRS <10). At 8 weeks post-infusion found that 33% of patients were in remission; of the responders at 2 weeks, 73% (8/11) remained in remission. [6]

References:

[1] Corriger A, Pickering G. Ketamine and depression: a narrative review. Drug Des Devel Ther. 2019;13:3051‐3067.
[2] Serafini G, Howland R, Rovedi F, Girardi P, Amore M. The Role of Ketamine in Treatment-Resistant Depression: A Systematic Review. Curr Neuropharmacol. 2014;12(5):444-461.
[3] Fond G, Loundou A, Rabu C, et al. Ketamine administration in depressive disorders: a systematic review and meta-analysis. Psychopharmacology (Berl). 2014;231(18):3663-76.
[4] McIntyre RS, Rosenblat JD, Nemeroff CB, et al. Synthesizing the Evidence for Ketamine and Esketamine in Treatment-Resistant Depression: An International Expert Opinion on the Available Evidence and Implementation. Am J Psychiatry. 2021;178(5):383-399. doi:10.1176/appi.ajp.2020.20081251
[5] Marcantoni WS, Akoumba BS, Wassef M, et al. A systematic review and meta-analysis of the efficacy of intravenous ketamine infusion for treatment resistant depression: January 2009 - January 2019. J Affect Disord. 2020;277:831-841. doi:10.1016/j.jad.2020.09.007
[6] Siegel JS, Palanca BJA, Ances BM, et al. Prolonged ketamine infusion modulates limbic connectivity and induces sustained remission of treatment-resistant depression. Psychopharmacology (Berl). 2021;238(4):1157-1169. doi:10.1007/s00213-021-05762-6

Literature Review

A search of the published medical literature revealed 5 studies investigating the researchable question:

How is IV ketamine administered for treatment-resistant depression?

Please see Tables 1-5 for your response.


 

Efficacy of ketamine therapy in the treatment of depression

Design

Open-label, prospective, cohort study 

N=20

Objective

To determine the effect of subanesthetic dose of ketamine on depressive and anxiety symptoms

Study Groups

Ketamine (N=20)

Methods

Inclusion criteria: male patients meeting ICD-10 diagnostic criteria for "severe depressive episode" with a Hamilton Rating Scale for Depression (HAM-D) score > 16, drug-free for at least 4 weeks (5 weeks for fluoxetine)

Exclusion criteria: any major comorbid psychiatric disorder, organic disorders, or substance use disorders except (nicotine or caffeine), electrocardiogram abnormality or abnormality on fundoscopy

Patients fit for ketamine injection were kept fasting for six hours. A ketamine subanesthetic dose of 0.5mg/kg was delivered as an IV bolus within two minutes. Five more doses were given (total 6 doses) on days 3, 6, 9, 12, and 14.

One hour after completion of the first dose of ketamine, HAM-D, Hamilton Anxiety Rating Scale (HAM-A), and clinical global impression (CGI) scales were administered. Twenty-four hours after the last session of IV ketamine, the scales were applied again.

Conventional treatment (antidepressants) was started at the end of the last dose of ketamine. One month after the last dose of ketamine an assessment irrespective of the antidepressants was made. 

Duration

Treatment duration: six doses in 2 weeks 

Follow up: 1 month after last ketamine dose

Outcome Measures

Effect of ketamine on psychopathy using HAM-D, Hamilton Anxiety Rating Scale (HAM-A), CGI scales

Baseline Characteristics

 

Ketamine (N=20)

 

Age, years

33.5 ± 8.80  

Age of onset of illness, years

29.90 ± 8.73  

Male

20 (100%)  

Duration of illness, months

6.95 ± 4.31   

Diagnosis

Moderate depressive episode without somatic syndrome

Moderate depressive episode with somatic syndrome

Recurrent depressive disorder with psychotic symptoms

Recurrent depressive disorder without psychotic symptoms

 

2 (10%)

7 (35%)

7 (35%)

4 (20%)

 

Antidepressant naive

10 (50%)

 

Results

 

Ketamine (N=20) P-value 

HAM-D score

Baseline

After 1st dose

After 2 weeks (all 6 doses)

After 1 month

 

23.40 ± 5.38

21.20 ± 5.38

10.25 ± 6.40 

10.45 ± 8.47 

 

 

0.003

<0.001

<0.001

HAM-A Score

Baseline

After 1st dose

After 2 weeks (all 6 doses)

After 1 month

 

20.00 ± 8.27

18.30 ± 6.73

8.90 ± 5.72

8.70 ± 7.09

 

 

0.027

<0.001

<0.001

CGI severity of illness score

Baseline

After 1st dose

After 2 weeks (all 6 doses)

After 1 month

 

4.20 ± 0.41

4.15 ± 0.49

2.40 ± 0.88

2.55 ± 1.28

 

 

0.330

<0.001

<0.001

HAM-D=Hamilton Rating Scale for Depression; HAM-A=Hamilton Rating Scale for Anxiety; CGI=Clinical Global severity of illness scale

Adverse Events

Mild transient adverse events were experienced within 1 hour of injection but were not reported afterward.

Study Author Conclusions

This provides substantiative evidence for ketamine's rapid and sustained effective role in depressive disorders and it's tolerability at a subanesthetic dose of 0.5mg/kg body weight by IV bolus injection. Ketamine has a robust and rapid effect on depression, which was seen immediately after the administration of ketamine and sustained at the end of 1 month.

InpharmD Researcher Critique

Some limitations of this study include the open-label and single cohort design, which limits the interpretation of efficacy. The small sample size and absence of the female sex also limits the generalizability of the study. 



References:

Mandal S, Sinha VK, Goyal N. Efficacy of ketamine therapy in the treatment of depression. Indian J Psychiatry. 2019;61(5):480‐485.

 

A Double-Blind, Randomized, Placebo-Controlled, Dose-Frequency Study of Intravenous Ketamine in Patients With Treatment-Resistant Depression

Design

Double-blind, randomized, placebo-controlled, parallel-group multicenter, open-label, phase II trial

N= 67

Objective

To evaluate the efficacy of two dosing regimens of ketamine at 0.5 mg/kg administered intravenously over 40 minutes at sustaining the antidepressant effects of ketamine in patients with treatment-resistant depression beyond the initial dose

Study Groups

Placebo two times/week (n=16)

Ketamine two times/week (n=18)

Placebo three times/week (n=16)

Ketamine three times/week (n=17)

Methods

Inclusion criteria: adults who met Diagnostic and Statistical Manual (DSM) IV criteria for recurrent major depressive disorder without psychotic features, confirmed by the Mini International Neuropsychiatric Interview; qualifying valid depressive episodes, as assessed with the SAFER criteria (defined as state versus trait, assessability, face validity, ecological validity, and rule of three Ps—pervasive, persistent, and pathological); inadequate response to at least two antidepressants (with at least one antidepressant failure in the current episode), assessed by medication history and the Massachusetts General Hospital Antidepressant Treatment Response Questionnaire; a score ≥34 on the 30-item Inventory of Depressive Symptomatology–Clinician Rated at screening and preinfusion assessment on day 1

Exclusion criteria: a primary DSM-IV diagnosis of obsessive-compulsive disorder, posttraumatic stress disorder, anorexia nervosa, or bulimia nervosa or a prior history or current diagnosis of a psychotic disorder, bipolar disorder, mental retardation, borderline personality disorder, mood disorder with postpartum onset, or somatoform disorders. Other exclusion criteria were a history of previous nonresponse of depressive symptoms to ketamine, clinically significant suicidal or homicidal ideation (imminent risk of harm), and substance abuse or dependence within the year preceding the screening visit

Patients randomized in a 1:1:1:1 ratio to one of the four treatment groups (ketamine or placebo either two or three times per week). Patients fasted overnight (≥8 hours) before study drug administration until two hours after the start of infusion. 

There were four phases: an up-to-four-week screening phase; a four-week double-blind treatment phase (day 1 to day 29); an optional two-week open-label treatment phase; and an up-to-three-week ketamine-free follow-up phase. An optional two-week open-label treatment of IV ketamine 0.5 mg/kg was offered to those who completed at least until day 15 but discontinued due to lack of efficacy before day 29.

Patients continued any antidepressant medications they were receiving at screening, at the same stable dosages throughout the study.

Duration

Each patient completed 13 weeks from July 2012 to September 2013

Outcome Measures

Primary outcome: change from baseline to day 15 in total Montgomery-Asberg Depression Rating Scale (MADRS) score

Secondary outcomes: common and serious adverse reactions

Baseline Characteristics

 

Twice-weekly dosing

Thrice weekly dosing

Placebo (n=16) Ketamine (n=18) Placebo (n=16) Placebo (n=17)

Age, years

40.3 ± 11.8 45.7 ± 9.6 46.1 ± 10.5 43.3 ± 12.0

Weight, kg

80.5 ± 19.7 80.6 ± 18.6 87.0 ± 23.7 75.9 ± 18.6

Body mass index, kg/m2

27.7 ± 6.4 29.3 ± 6.5 28.8 ± 5.8 26.6 ± 6.2

Baseline MADRS score

35.6 ± 3.8 33.3 ± 4.9 36.8 ± 5.8  35.4 ± 5.3

Women

12 (75%) 12 (66.7%) 9 (56.3%) 12 (70.6%)

White

15 (93.8%) 12 (66.7%) 15 (93.8%) 64.7 (53%)

Number of antidepressants in current episode

1

2

3

4

≥5

 

6 (37.5%)

9 (56.3%)

1 (6.3%)

0 (0%)

0 (0%)

 

9 (50%)

6 (33.3%)

1 (5.6%)

2 (11.1%)

0 (0%)

 

10 (62.5%)

2 (12.5%)

2 (12.5%)

0 (0%)

2 (12.5%)

 

11 (64.7%)

4 (23.5%)

1 (5.9%)

0 (0%)

1 (5.9%)

Results

 

Twice weekly dosing

Thrice weekly dosing

Placebo (n=16) Ketamine (n=18) Placebo (n=16) Ketamine (n=17)

Mean change in MADRS score from baseline to day 15

-5.7 ± 10.2

-18.4 ± 12*

-3.1 ± 5.7

-17.7 ± 7.3*

Common adverse events

Headaches

Anxiety

Dissociation

Nausea

Dizziness

 

5 (31.3%)

0 (0%)

0 (0%)

1 (6.3%)

1 (6.3%)

 

4 (22.2%)

5 (27.8%)

5 (27.8%)

3 (16.7%)

4 (22.2%)

 

1 (6.3%)

0 (0%)

0 (0%)

2 (12.5%)

0 (0%)

 

7 (41.2%)

1 (5.9%)

1 (5.9%)

4 (23.5%)

2 (11.8%)

Serious adverse events

Anxiety 

Suicide attempt 

 

0 (0%)

0 (0%)

 

1 (5.6%)

1 (5.6%)

 

0 (0%)

0 (0%)

 

0 (0%)

0 (0%)

Discontinued study due to Adverse Event 

1 (6.3%)

4 (22%)

0 (0%)

4 (23%)

*P<0.001; MADRS= Montgomery-Åsberg Depression Rating Scale

Study Author Conclusions

Twice-weekly and thrice-weekly administration of ketamine at 0.5 mg/kg similarly maintained antidepressant efficacy over 15 days. 

InpharmD Researcher Critique

Intravenous ketamine when given twice or three times weekly can be used for treatment-resistant depression with common adverse events such as headaches and anxiety. This study was a double-blind, parallel-group, and randomized with all groups having similar baseline characteristics. One limitation is the short duration of 15 days follow-up. More time could better assess if the clinical benefits can be maintained. 



References:

Singh J, Fedgchin M, Daly E et al. A Double-Blind, Randomized, Placebo-Controlled, Dose-Frequency Study of Intravenous Ketamine in Patients With Treatment-Resistant Depression. American Journal of Psychiatry. 2016;173(8):816-826.

 

Double-blind, placebo-controlled, dose-ranging trial of intravenous ketamine as adjunctive therapy in treatment-resistant depression (TRD)

Design

Double-blind, placebo-controlled study

N=99

Objective

To compare to active placebo the rapid acting antidepressant properties of a broad range of subanesthetic doses of IV ketamine among outpatients with treatment-resistant depression (TRD) 

Study Groups

Ketamine 0.1 mg/kg (n=18)

Ketamine 0.2 mg/kg (n=20)

Ketamine 0.5 mg/kg (n=22)

Ketamine 1 mg/kg (n=20)

Midazolam 0.045 mg/kg (n=19)

Methods

Inclusion criteria: adults aged 18-70 years with a diagnosis of major depressive disorder (MDD) in a current depressive episode of at least 8 weeks, as defined by the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition-Text Revision (DSM-IV-TR); had treatment-resistant depression (TRD), defined as failure to achieve a subjective satisfactory response (less than 50% improvement of depression symptoms) to at least two adequate treatment courses during the current depressive episode, including the current antidepressant; were on a stable (for at least four weeks) and adequate dose of ongoing antidepressant therapy, with a total treatment duration of at least 8 weeks; BMI between 18-35 kg/m2; a Montgomery-Asberg Depression Rating Scale (MADRS) score of ≥20 at both screening and baseline visits.

Exclusion criteria: failure to achieve satisfactory response (less than 50% improvement of depression symptoms) to >7 treatment courses of a therapeutic dose of an antidepressant of at least eight weeks in the current major depressive episode; MADRS total score of <20 at screening or baseline; a primary Axis I disorder other than MDD; current substance use disorder (abuse or dependence), with the exception of nicotine dependence, within six months of screening; a history of ketamine or phencyclidine drug use; a history of bipolar disorder, schizophrenia, or schizoaffective disorders; a history of psychotic symptoms in the current or previous depressive episodes; previous participants in research studies involving glutamatergic agents for depression

Following a washout period for patients on prohibited psychotropic agents, eligible subjects were randomly assigned to one of five 40 minute infusion arms in a 1:1:1:1:1 fashion. Prior to randomization, patients were grouped by body mass index (BMI) (group I: BMI≤ 30; group II: BMI>30) and were block randomized into each arm of the study, with the mg/kg ratio being maintained across all BMIs.

Duration

Follow-up: 30 days

Outcome Measures

Primary outcome: change in 6-item Hamilton Depression Rating Scale (HAM-D-6) score  within 72 hours of ketamine, when added to stable antidepressant therapy

Secondary outcomes: MADRS, self-rated Symptoms of Depression Questionnaire (SDQ), the self-rated Positive Affect Scale (PAS), and global severity and improvement scales of the Clinical Global Impressions (CGI-S) 

Baseline Characteristics

 

Ketamine 0.1 mg/kg (n=18)

Ketamine 0.2 mg/kg  (n=20)

Ketamine 0.5 mg/kg (n=22)

Keatmine 1 mg/kg (n =20)

Midazolam 0.045 mg/kg (n =19)

Age, years

43.1±11.9 45.5±14.6 48.6±12.9 47.4±10.1 45.6±13.8

BMI, kg/m2

25.2±3.1 24.9±3.7 25.3±5.7 26.1±3.8 26.3±4.1

Female

55.6% 45% 50% 40% 57.9%

White race

 

66.7%

100%

90.9%

90%

94.7%

Concomitant medications

Benzodiazepines

SSRIs

SNRIs

TCAs

Other antidpressant(s)

 

50%

44.4%

27.8%

5.6%

44.4%

 

50%

60%

35%

0

55%

 

45.5%

54.6%

31.8%

4.6%

59.1%

 

45%

50%

20%

5%

40%

 

31.6%

52.6%

26.3%

0

57.9%

Clinical severity at baseline

Failed antidepressants for current episode

HAM-D-6 score

MADRS score

 

3.3±1.3

 

12.6±1.8

33.8±5.9

 

3.7±1.6

 

12.8±2.5

34.5±8.5

 

2.7±1.2

 

12.6±1.5

31.6±3.9

 

2.9±1.2

 

12.6±2.1

32.7±5.9

 

2.9±1.4

 

13.1±2.3

33.6±7.1

BMI, body mass index; SSRIs, selective serotonin reuptake inhibitors; SNRIs, serotonin-norepinephrine reuptake inhibitors; TCAs, tricyclic antidepressants; HAM-D-6, Hamilton Rating Scale for Depression, 6-item version; MADRS, Montgomery-Asberg Depression Rating Scale 

Results

  Ketamine 0.1 mg/kg (n=14) Ketamine 0.2 mg/kg (n=16) Ketamine 0.5 mg/kg (n=21) Ketamine 1.0 mg/kg (n=17)  

Change in HAM-D-6 score

Day 1

Day 3

 

-3.18

-2.04

 

-1.13

-0.36

 

-4.79*

-3.21

 

-3.76*

-1.84

 
Change in MADRS score after day 3 -5.15 -2.16

-9.85*

-7.72  
*P<0.05

Adverse Events

Common Adverse Events: headache (11.3% vs 0%), nausea (10% vs 0%), vomiting (5% vs 0%), and depression (3.8% vs 0%)

High systolic blood pressure readings occurred in a total of 21 participants in the ketamine groups, and high diastolic blood pressure readings occurred in a total of 10 participants in the ketamine groups; at 40 minutes after infusion start, both 0.5 mg/kg and 1 mg/kg doses had significantly greater CADSS (Clinician-Administered Dissociative States Scale) scores than active placebo.

Serious Adverse Events: Spontaneously reported suicidal ideation was reported by two ketamine-treated patients, but none of the active-placebo-treated patients; one participant attempted suicide by overdosing on day 11 (received ketamine 0.2 mg/kg in study).

Study Author Conclusions

Results suggest evidence for the efficacy of the 0.5 mg/kg and 1.0 mg/kg subanesthetic doses of IV ketamine and no clear or consistent evidence for clinically meaningful efficacy of lower doses of IV ketamine.

InpharmD Researcher Critique

Treatment groups had small sample sizes, and results may have been confounded by the variability in the degree of responsiveness to ketamine across the treatment groups. This study does not answer the question of whether raising doses in poor responders to the standard dose of 0.5 mg/kg of ketamine is helpful and tolerated, or if lower doses are effective in patients who cannot tolerate the standard ketamine dose of 0.5 mg/kg.  Of this small group, two patients with suicidal ideation and one attempted suicide is noteworthy. 

Additionally, this study had a short duration. Although patients were followed for up to 30 days, their HAM-D and MADRS scores were not assessed at day 30. The midazolam group never had their HAM-D or MADRS scores reported, so there is no comparison to placebo.



References:

Fava M, Freeman MP, Flynn M, et al. Double-blind, placebo-controlled, dose-ranging trial of intravenous ketamine as adjunctive therapy in treatment-resistant depression (TRD). Mol Psychiatry. 2018.

 

Antidepressant efficacy of ketamine in treatment-resistant major depression: a two-site randomized controlled trial

Design

Two-site, parallel-arm, randomized, controlled trial

N=73

Objective

To evaluate the rapid antidepressant efficacy of ketamine in a large group of patients with treatment-resistant major depression

Study Groups

Ketamine (n=47)

Midazolam (n=25)

Methods

Inclusion criteria: patients 21 to 80 years old with a primary diagnosis of major depressive disorder as assessed with the Structured Clinical Interview for DSM-IV (Diagnostic and Statistical Manual of Mental Disorders, fourth edition), and an inadequate response to at least three therapeutic trials of an antidepressant according to the criteria of the Antidepressant Treatment History Form; a history of at least one previous major depressive episode prior to the current episode (recurrent major depressive disorder) or the combination of a chronic major depressive episode (at least two years' duration) and a score of 32 or greater on the Inventory of Depressive Symptomatology

Exclusion criteria: patients who had a lifetime history of a psychotic illness or bipolar disorder, alcohol or substance abuse in the previous two years, unstable medical illness, serious and imminent suicidal or homicidal risk, a score less than 27 on the Mini-Mental State Examination, or if they were taking contraindicated medications

Randomly assigned in a 2:1 ratio, patients received a single intravenous infusion of ketamine hydrochloride (0.5 mg/kg) or midazolam (0.045 mg/kg) infused over 40 minutes. Patients were discharged from the research unit 24 hours after the infusion and received outpatient evaluation 48 hours, 72 hours, and seven days post-infusion.

Patients were instructed to abstain from psychotropic medications and to abstain from substances of abuse and alcohol while at home. Nonresponders were considered patients with less than 50% improvement from baseline in the score on the Montgomery-Asberg Depression Rating Scale (MADRS). Nonresponders were no longer followed seven days after the infusion. Responders were followed biweekly until relapse or for an additional four weeks, whichever came sooner.

Duration

Follow-up: at least 7 days post-infusion

Outcome Measures

Primary outcome: reduction in depression severity as assessed on the clinician-administered MADRS 24 hours following infusion

Secondary outcomes: MADRS response rate (defined as reduction in the baseline score by 50% or more), change in score on the Quick Inventory of Depressive Symptomatology, scores on the Clinical Global Improvement (CGI) severity and improvement measures, and durability of benefit for up to 7 days following infusion

Baseline Characteristics

 

Ketamine (n=47)

Midazolam (n=25)

Age, years

46.9 ± 12.8 42.7 ± 11.6

Female

26 (55%) 11 (44%)

White

37 (79%) 23 (92%)
Recurrent major depressive disorder 28 (60%) 16 (64%)
Chronic index episode (lasting ≥2 years) 33 (70%) 16 (64%)
Duration of index episode, months 146.6 ± 158.3 109.2 ± 139.0
Previous antidepressant failures 5.1 ± 2.0 5.0 ± 1.8
Montgomery-Asberg Depression Rating Scale score 32.6 ± 6.1 31.1 ± 5.6
16-item Quick Inventory of Depressive Symptomatology score 16.6 ± 4.1 16.3 ± 4.5

Results

 

Ketamine (n=47)

Midazolam (n=25)

MADRS score (95% CI)

After 24 hours

After 7 days

 

14.77 (11.73-17.80)

17.85 (14.29-21.42)

 

22.72 (18.85-26.59)

23.54 (18.29-28.79

Quick Inventory of Depressive Symptomatology-Self Report score (95% CI)

After 24 hours

After 7 days

 

8.38 (6.71-10.05)

8.58 (6.92-10.24)

 

11.78 (9.63-13.92)

11.42 (8.87-13.97)

≥50% decrease in MADRS score after 24 hours 30 (64%) 7 (28%)

Clinical Global Impression Scale

Improvement rating of 2 (much improved) or 1 (very much improved)

Severity rating of 2 (minimally ill) or 1 (not at all ill)

 

29 (62%)

25 (53%)

 

6 (24%)

2 (8%)

Linear modeling showed that patients had significantly lower MADRS scores at day 7 with ketamine compared to midazolam (P≤0.02).

Adverse Events

Common Adverse Events: 

  • Most common in ketamine group within four hours of infusion (ketamine vs midazolam): dizziness (45% vs 20%), blurred vision (43% vs 8%), headache (32% vs 20%), nausea or vomiting (34% vs 12%), dry mouth (26% vs 16%), poor coordination (26% vs 12%), poor concentration (26% vs 8%), restlessness (21% vs 20%)
  • Most common adverse events in the midazolam group within four hours of infusion (ketamine vs midazolam): general malaise (6% vs 28%), dizziness (45% vs 20%), headache (32% vs 20%), restlessness (21% vs 20%), nausea or vomiting (34% vs 12%), dry mouth (26% vs 16%), decreased energy (15% vs 12%), poor coordination (26% vs 12%)
  • Dissociative symptoms were observed immediately after ketamine infusion in 8 patients receiving ketamine (17%).

Serious Adverse Events: No severe psychotic symptoms (paranoia, hallucinations, delusions, or thought disorders) or other serious events were noted in any patient.

Percentage that Discontinued due to Adverse Events: Treatment was discontinued for two patients in the ketamine group because of hemodynamic changes (4.2%).

Study Author Conclusions

Treatment-resistant patients in a major depressive episode showed a rapid antidepressant response to a single infusion of ketamine.

InpharmD Researcher Critique

There was stringent enrollment criteria due to concerns about ketamine's psychoactive effects and abuse liability leading to small sample sizes. The follow-up was also relatively short term, with the study only being powered to detect a major difference after 24 hours. There was no report of patients who were followed longer than 7 days.



References:

Murrough JW, Iosifescu DV, Chang LC, et al. Antidepressant efficacy of ketamine in treatment-resistant major depression: a two-site randomized controlled trial. Am J Psychiatry. 2013;170(10):1134‐1142.

 

7T 1H-MRS in major depressive disorder: a Ketamine Treatment Study

Design

Randomized, double-blind, placebo-controlled, crossover study

N=37

Objective

To assess the effects of ketamine on mood and brain glutamate changes measured at baseline and at 24 h post ketamine/post placebo infusion

Study Groups

Healthy volunteers (n=17)

Medication-free major depressive disorder [MDD] subjects (n=20)

Methods

Inclusion criteria: Adults ages 18 to 65, physically healthy as determined by medical history, physical exam, blood labs, urinalysis, and toxicology, free of any serious medical conditions, comorbid substance abuse, and lifetime dependence (excluding caffeine and nicotine). All MDD subjects were currently experiencing a major depressive episode lasting ≥4 weeks and had been medication-free for at least 2 weeks prior to study randomization. Additionally, a Montgomery-Åsberg Depression Rating Scale (MADRS) score ≥20 at the time of screening and also prior to each infusion was an inclusion criterion for MDD subjects. MDD subjects were refractory to pharmacological treatment (treatment resistance was defined as not having responded to at least one adequate antidepressant dose/duration trial).

Exclusion criteria: healthy volunteers that had ay first-degree relatives diagnosed with a major psychiatric disorder

Participants randomly received one IV infusion of ketamine hydrochloride 0.5mg/kg or one infusion of placebo (normal saline). Two weeks later, participants received the other infusion. Twenty-four hours after the infusions, participants received a 7T magnetic resonance imaging (MRI) scan and 1H-MRS (proton magnetic resonance spectroscopy).

Duration

24 hours

Outcome Measures

Primary outcome: glutamate levels at baseline and at 24 h post ketamine and post-placebo infusions via 1H-MRS (proton magnetic resonance spectroscopy) scans at 7T

Secondary outcome: additional metabolite changes, changes in mood rating scales

Baseline Characteristics

 

Healthy volunteers (n=17)

Major depressive disorder (n=20)

 

Age, years

34.7 ± 2.9 36.2 ± 2.5  

Female

12 (71%) 12 (60%)  
Body mass index, kg/m2 27.0 ± 1.0 27.5 ± 1.5  
Length of illness, years --- 21 ± 2.7  
Current episode, months --- 47 ± 17.3  

Results

 

Healthy volunteers (n=17)

Major depressive disorder (n=20)

P-value

Glutamate

Baseline

Ketamine

Placebo

 

1.25 ± 0.02

1.28 ± 0.03

1.27 ± 0.03

 

1.29 ± 0.02

1.36 ± 0.02

1.32 ± 0.02

 

0.7

0.7

0.7 

Glutamine

Baseline

Ketamine

Placebo

 

0.29 ± 0.02

0.31 ± 0.03

0.28 ± 0.03

 

0.29 ± 0.02

0.30 ± 0.02

0.31 ± 0.02

 

NS

NS

NS

MADRS score

Baseline

After ketamine

After placebo

 

1.2 ± 0.3

2.3 ± 0.8

0.7 ± 0.4

 

32.9 ± 1.1

22.9 ± 2.3

30.9 ± 1.1

N/A 

Ham-D score

Baseline

After ketamine

After placebo

 

1.42 ± 0.3

1.54 ± 0.5

0.25 ± 0.2

 

21.8 ± 1.1

15.6 ± 1.5

18.7 ± 0.9

N/A
MADRS = Montgomery-Åsberg Depression Rating Scale, HAM-D = Hamilton Depression Rating Scale

Adverse Events

Not studied

Study Author Conclusions

This study investigated glutamate and glutamine levels in the pregenual anterior cingulate cortex (pgACC) in both unmedicated, treatment-resistant MDD subjects and healthy volunteers at baseline and at 24 h after a single infusion of intravenous ketamine and placebo. No significant differences in metabolites, either between groups or between scan sessions, were seen. In addition, no correlations were observed between glutamate levels and mood, and baseline glutamate levels were not associated with subsequent response to ketamine.

They found that 7T 1H-MRS pgACC glutamate differences between HVs and MDD subjects in this study were less than the measurement sensitivity of the pulse sequence (~8% change). The data suggest that larger effects may occur earlier than 24 h or in a different brain region, or that subgroups of MDD subjects may exist that have a differential response to ketamine. Their findings may help guide future investigations of the neurochemical changes post ketamine administration in individuals with MDD.

InpharmD Researcher Critique

This study had a small sample size, which limited the ability to draw conclusions from their results. The primary endpoint was based on surrogate outcomes instead of clinical endpoints (such as mood changes). A time-fram of 24 hours after infusion may also have been too short to detect major differences. Only one area of the brain (the pregenual anterior cingulate cortex) was focused; there could have been significant changes in other parts of the brain. Additionally, the study chose to reference metabolite values to creatine, which could lead to variance due to disease-specific or individual-specific factors.



References:

Evans JW, Lally N, An L, et al. 7T H-MRS in major depressive disorder: a Ketamine Treatment Study. Neuropsychopharmacology. 2018;43(9):1908-1914.